Abstract
Multiple Sclerosis (MS) is a highly heterogeneous disease in terms of clinical and
paraclinical aspects, partly attributed to the possible differences in environmental and genetic aetiology. Oligoclonal bands (OCB) are an intrathecal IgG response which can be visualized by isoelectric focusing (IEF) and immunoblotting. OCB has been reported to be important in determining the immunopathology of MS and disease progression.
The objective of this study is to compare the OCB numbers in Western and Sri
Lankan MS patients.
Serum and CSF electrophoresis was performed using the horizontal bed
electrophoresis system in a pH gradient. The standard IEF protocol followed in Karolinska Hospital, Sweden was adapted to suit our laboratory conditions. OCB positivity or negativity band counts were independently observed and recorded by three trained investigators. Two or more OCBs present in CSF and absent in serum were considered as positive for MS. Statistical significance of the difference of the mean OCB counts was calculated by Unpaired t
test using Graph pad prism 5 software.
Statistical analysis confirmed that the mean values of average OCB counts in western positive controls were significantly higher than the Sri Lankan OCB positive MS patients (P < 0.05).
Thus, a significant quantitative difference is observed in oligoclonal antibody
response between prototypic MS in western and Sri Lankan MS patients. This may have an association to the differences in immunopathology and clinical subtypes of MS in different geographical regions. However, a larger sample is required to further confirm these observations.
paraclinical aspects, partly attributed to the possible differences in environmental and genetic aetiology. Oligoclonal bands (OCB) are an intrathecal IgG response which can be visualized by isoelectric focusing (IEF) and immunoblotting. OCB has been reported to be important in determining the immunopathology of MS and disease progression.
The objective of this study is to compare the OCB numbers in Western and Sri
Lankan MS patients.
Serum and CSF electrophoresis was performed using the horizontal bed
electrophoresis system in a pH gradient. The standard IEF protocol followed in Karolinska Hospital, Sweden was adapted to suit our laboratory conditions. OCB positivity or negativity band counts were independently observed and recorded by three trained investigators. Two or more OCBs present in CSF and absent in serum were considered as positive for MS. Statistical significance of the difference of the mean OCB counts was calculated by Unpaired t
test using Graph pad prism 5 software.
Statistical analysis confirmed that the mean values of average OCB counts in western positive controls were significantly higher than the Sri Lankan OCB positive MS patients (P < 0.05).
Thus, a significant quantitative difference is observed in oligoclonal antibody
response between prototypic MS in western and Sri Lankan MS patients. This may have an association to the differences in immunopathology and clinical subtypes of MS in different geographical regions. However, a larger sample is required to further confirm these observations.
Original language | English |
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Pages | 164-164 |
Number of pages | 1 |
Publication status | Published - 4 Nov 2016 |
Externally published | Yes |
Event | PERADENIYA UNIVERSITY INTERNATIONAL RESEARCH SESSIONS: iPURSE 2016 - University of Peradeniya, Sri Lanka Duration: 4 Nov 2016 → 5 Nov 2016 https://site.pdn.ac.lk/ipurse/2016/ (2016 conference website) |
Conference
Conference | PERADENIYA UNIVERSITY INTERNATIONAL RESEARCH SESSIONS: iPURSE 2016 |
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Abbreviated title | iPURSE 2016 |
Country/Territory | Sri Lanka |
Period | 4/11/16 → 5/11/16 |
Other | iPURSE-2016,the Annual International Research Sessions of the University of Peradeniya, hosted by the Faculty of Dental Sciences will be held on 4th and 5th November 2016. The University of Peradeniya, nestled in the foothills of the misty Hantana mountain range is a premier academic institution in Sri Lanka renowned for excellence in teaching and research. Located on the banks of the river Mahaweli, it is in close proximity to the Royal Botanical Gardens and the historic city of Kandy. iPURSE-2016 aims to provide an open forum for researchers from different disciplines to deliberate on their research findings, exchange ideas, build partnerships and collaborations for the advancement of knowledge.. |
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