Comparing self-assembling and covalent fluorescent boronolectins for the detection of free sialic acid

Stephan M. Levonis, Milton J. Kiefel, Todd A. Houston*

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

6 Citations (Scopus)

Abstract

A self-assembling fluorescence sensor with boronic acid functionalities was tested for binding selectivity to the monosaccharide, sialic acid. Working from a previously reported system, a self-assembling system could form an imine in situ that enables a conjugated fluorophore to display a measurable change in fluorescence in the presence of monosaccharide. However, further examination showed that free sugars give a similar fluorescence response to just the m-aminophenylboronic acid moiety on its own. Still, such a self-assembly method may be applicable to cell surface saccharide sensing as aldehydes and ketones are noticeably absent on most cells' exteriors. The original covalent receptor appears best suited for the detection of free sialic acid.

Original languageEnglish
Pages (from-to)1454-1457
Number of pages4
JournalAustralian Journal of Chemistry
Volume64
Issue number11
DOIs
Publication statusPublished - 2011
Externally publishedYes

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