Circulating microrna responses between 'high' and 'low' responders to a 16-Wk diet and exercise weight loss intervention

Evelyn B. Parr, Donny M. Camera, Louise M. Burke, Stuart M. Phillips, Vernon G. Coffey, John A. Hawley

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Abstract

Background: Interactions between diet, physical activity and genetic predisposition contribute to variable body mass changes observed in response to weight loss interventions. Circulating micro-RNAs (c-miRNAs) may act as 'biomarkers' that are associated with the rate of change in weight loss, and/or play a role in regulating the biological variation, in response to energy restriction. Objective: To quantify targeted c-miRNAs with putative roles in energy metabolism and exercise adaptations following a 16 wk diet and exercise intervention in individuals with large (high responders; HiRes) versus small (low responders; LoRes) losses in body mass. Methods: From 89 male and female overweight/obese participants who completed the intervention (energy restriction from diet, 250 kcal/d, and exercise, 250 kcal/d), subgroups of HiRes (>10% body mass loss, n = 22) and LoRes (<5% body mass loss, n = 18) were identified. From resting plasma samples collected after an overnight fast pre and post intervention, RNA was extracted, quantified and reverse transcribed. Thirteen c-miRNA selected a priori were analysed using a customised 96-well miScript miRNA PCR Array. Results: Loss of body mass (-11.0 ± 2.3 kg vs. -3.0 ± 1.3 kg; P<0.01) and fat mass (-11.1 ± 2.6 kg vs. -3.9 ± 1.6 kg; P<0.01) was greater for HiRes than LoRes (P<0.001). Expression of c-miR-935 was higher in LoRes compared to HiRes pre-(∼47%; P = 0.025) and post-(∼100%; P<0.01) intervention and was the only c-miRNA differentially expressed at baseline between groups. The abundance of c-miR-221-3p and -223-3p increased pre- to post-intervention in both groups (∼57-69% and ∼25-90%, P<0.05). There was a post-intervention increase in c-miR-140 only in LoRes compared to HiRes (∼23%, P = 0.016). Conclusion The differential expression and responses of selected c-miRNAs in overweight/obese individuals to an exercise and diet intervention suggests a putative role for these 'biomarkers' in the prediction or detection of individual variability to weight loss interventions.

Original languageEnglish
Article numbere0152545
JournalPLoS One
Volume11
Issue number4
DOIs
Publication statusPublished - 1 Apr 2016

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Reducing Diet
Nutrition
MicroRNAs
microRNA
exercise
weight loss
diet
Diet
Weight Loss
Biomarkers
biomarkers
energy
Genetic Predisposition to Disease
physical activity
energy metabolism
Energy Metabolism
Fats
RNA
Polymerase Chain Reaction
prediction

Cite this

Parr, Evelyn B. ; Camera, Donny M. ; Burke, Louise M. ; Phillips, Stuart M. ; Coffey, Vernon G. ; Hawley, John A. / Circulating microrna responses between 'high' and 'low' responders to a 16-Wk diet and exercise weight loss intervention. In: PLoS One. 2016 ; Vol. 11, No. 4.
@article{e81e70195bb44e7196453ce90cad595e,
title = "Circulating microrna responses between 'high' and 'low' responders to a 16-Wk diet and exercise weight loss intervention",
abstract = "Background: Interactions between diet, physical activity and genetic predisposition contribute to variable body mass changes observed in response to weight loss interventions. Circulating micro-RNAs (c-miRNAs) may act as 'biomarkers' that are associated with the rate of change in weight loss, and/or play a role in regulating the biological variation, in response to energy restriction. Objective: To quantify targeted c-miRNAs with putative roles in energy metabolism and exercise adaptations following a 16 wk diet and exercise intervention in individuals with large (high responders; HiRes) versus small (low responders; LoRes) losses in body mass. Methods: From 89 male and female overweight/obese participants who completed the intervention (energy restriction from diet, 250 kcal/d, and exercise, 250 kcal/d), subgroups of HiRes (>10{\%} body mass loss, n = 22) and LoRes (<5{\%} body mass loss, n = 18) were identified. From resting plasma samples collected after an overnight fast pre and post intervention, RNA was extracted, quantified and reverse transcribed. Thirteen c-miRNA selected a priori were analysed using a customised 96-well miScript miRNA PCR Array. Results: Loss of body mass (-11.0 ± 2.3 kg vs. -3.0 ± 1.3 kg; P<0.01) and fat mass (-11.1 ± 2.6 kg vs. -3.9 ± 1.6 kg; P<0.01) was greater for HiRes than LoRes (P<0.001). Expression of c-miR-935 was higher in LoRes compared to HiRes pre-(∼47{\%}; P = 0.025) and post-(∼100{\%}; P<0.01) intervention and was the only c-miRNA differentially expressed at baseline between groups. The abundance of c-miR-221-3p and -223-3p increased pre- to post-intervention in both groups (∼57-69{\%} and ∼25-90{\%}, P<0.05). There was a post-intervention increase in c-miR-140 only in LoRes compared to HiRes (∼23{\%}, P = 0.016). Conclusion The differential expression and responses of selected c-miRNAs in overweight/obese individuals to an exercise and diet intervention suggests a putative role for these 'biomarkers' in the prediction or detection of individual variability to weight loss interventions.",
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Circulating microrna responses between 'high' and 'low' responders to a 16-Wk diet and exercise weight loss intervention. / Parr, Evelyn B.; Camera, Donny M.; Burke, Louise M.; Phillips, Stuart M.; Coffey, Vernon G.; Hawley, John A.

In: PLoS One, Vol. 11, No. 4, e0152545, 01.04.2016.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Circulating microrna responses between 'high' and 'low' responders to a 16-Wk diet and exercise weight loss intervention

AU - Parr, Evelyn B.

AU - Camera, Donny M.

AU - Burke, Louise M.

AU - Phillips, Stuart M.

AU - Coffey, Vernon G.

AU - Hawley, John A.

PY - 2016/4/1

Y1 - 2016/4/1

N2 - Background: Interactions between diet, physical activity and genetic predisposition contribute to variable body mass changes observed in response to weight loss interventions. Circulating micro-RNAs (c-miRNAs) may act as 'biomarkers' that are associated with the rate of change in weight loss, and/or play a role in regulating the biological variation, in response to energy restriction. Objective: To quantify targeted c-miRNAs with putative roles in energy metabolism and exercise adaptations following a 16 wk diet and exercise intervention in individuals with large (high responders; HiRes) versus small (low responders; LoRes) losses in body mass. Methods: From 89 male and female overweight/obese participants who completed the intervention (energy restriction from diet, 250 kcal/d, and exercise, 250 kcal/d), subgroups of HiRes (>10% body mass loss, n = 22) and LoRes (<5% body mass loss, n = 18) were identified. From resting plasma samples collected after an overnight fast pre and post intervention, RNA was extracted, quantified and reverse transcribed. Thirteen c-miRNA selected a priori were analysed using a customised 96-well miScript miRNA PCR Array. Results: Loss of body mass (-11.0 ± 2.3 kg vs. -3.0 ± 1.3 kg; P<0.01) and fat mass (-11.1 ± 2.6 kg vs. -3.9 ± 1.6 kg; P<0.01) was greater for HiRes than LoRes (P<0.001). Expression of c-miR-935 was higher in LoRes compared to HiRes pre-(∼47%; P = 0.025) and post-(∼100%; P<0.01) intervention and was the only c-miRNA differentially expressed at baseline between groups. The abundance of c-miR-221-3p and -223-3p increased pre- to post-intervention in both groups (∼57-69% and ∼25-90%, P<0.05). There was a post-intervention increase in c-miR-140 only in LoRes compared to HiRes (∼23%, P = 0.016). Conclusion The differential expression and responses of selected c-miRNAs in overweight/obese individuals to an exercise and diet intervention suggests a putative role for these 'biomarkers' in the prediction or detection of individual variability to weight loss interventions.

AB - Background: Interactions between diet, physical activity and genetic predisposition contribute to variable body mass changes observed in response to weight loss interventions. Circulating micro-RNAs (c-miRNAs) may act as 'biomarkers' that are associated with the rate of change in weight loss, and/or play a role in regulating the biological variation, in response to energy restriction. Objective: To quantify targeted c-miRNAs with putative roles in energy metabolism and exercise adaptations following a 16 wk diet and exercise intervention in individuals with large (high responders; HiRes) versus small (low responders; LoRes) losses in body mass. Methods: From 89 male and female overweight/obese participants who completed the intervention (energy restriction from diet, 250 kcal/d, and exercise, 250 kcal/d), subgroups of HiRes (>10% body mass loss, n = 22) and LoRes (<5% body mass loss, n = 18) were identified. From resting plasma samples collected after an overnight fast pre and post intervention, RNA was extracted, quantified and reverse transcribed. Thirteen c-miRNA selected a priori were analysed using a customised 96-well miScript miRNA PCR Array. Results: Loss of body mass (-11.0 ± 2.3 kg vs. -3.0 ± 1.3 kg; P<0.01) and fat mass (-11.1 ± 2.6 kg vs. -3.9 ± 1.6 kg; P<0.01) was greater for HiRes than LoRes (P<0.001). Expression of c-miR-935 was higher in LoRes compared to HiRes pre-(∼47%; P = 0.025) and post-(∼100%; P<0.01) intervention and was the only c-miRNA differentially expressed at baseline between groups. The abundance of c-miR-221-3p and -223-3p increased pre- to post-intervention in both groups (∼57-69% and ∼25-90%, P<0.05). There was a post-intervention increase in c-miR-140 only in LoRes compared to HiRes (∼23%, P = 0.016). Conclusion The differential expression and responses of selected c-miRNAs in overweight/obese individuals to an exercise and diet intervention suggests a putative role for these 'biomarkers' in the prediction or detection of individual variability to weight loss interventions.

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