Characterization of the effect of LPS on dendritic cell subset discrimination in spleen

Kristin L Griffiths, Jonathan K H Tan, Helen C O'Neill

Research output: Contribution to journalArticleResearchpeer-review

6 Citations (Scopus)

Abstract

The Gram-negative bacterial endotoxin lipopolysaccharide (LPS) is a potent inflammatory mediator and a leading cause of bacterial sepsis. While LPS is known to activate antigen-presenting cells, here we find that LPS down-regulates expression of CD11c and CD11b on splenic dendritic cell subsets, thus confounding the ability to identify these subsets following treatment. This has implications with regard to tracking the response to LPS in terms of the cell subsets involved, and should be considered whenever such studies are undertaken.

Original languageEnglish
Pages (from-to)1908-12
Number of pages5
JournalJournal of Cellular and Molecular Medicine
Volume18
Issue number9
DOIs
Publication statusPublished - Sep 2014
Externally publishedYes

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Dendritic Cells
Lipopolysaccharides
Spleen
Antigen-Presenting Cells
Endotoxins
Sepsis
Down-Regulation

Cite this

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abstract = "The Gram-negative bacterial endotoxin lipopolysaccharide (LPS) is a potent inflammatory mediator and a leading cause of bacterial sepsis. While LPS is known to activate antigen-presenting cells, here we find that LPS down-regulates expression of CD11c and CD11b on splenic dendritic cell subsets, thus confounding the ability to identify these subsets following treatment. This has implications with regard to tracking the response to LPS in terms of the cell subsets involved, and should be considered whenever such studies are undertaken.",
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Characterization of the effect of LPS on dendritic cell subset discrimination in spleen. / Griffiths, Kristin L; Tan, Jonathan K H; O'Neill, Helen C.

In: Journal of Cellular and Molecular Medicine, Vol. 18, No. 9, 09.2014, p. 1908-12.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Characterization of the effect of LPS on dendritic cell subset discrimination in spleen

AU - Griffiths, Kristin L

AU - Tan, Jonathan K H

AU - O'Neill, Helen C

N1 - © 2014 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

PY - 2014/9

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N2 - The Gram-negative bacterial endotoxin lipopolysaccharide (LPS) is a potent inflammatory mediator and a leading cause of bacterial sepsis. While LPS is known to activate antigen-presenting cells, here we find that LPS down-regulates expression of CD11c and CD11b on splenic dendritic cell subsets, thus confounding the ability to identify these subsets following treatment. This has implications with regard to tracking the response to LPS in terms of the cell subsets involved, and should be considered whenever such studies are undertaken.

AB - The Gram-negative bacterial endotoxin lipopolysaccharide (LPS) is a potent inflammatory mediator and a leading cause of bacterial sepsis. While LPS is known to activate antigen-presenting cells, here we find that LPS down-regulates expression of CD11c and CD11b on splenic dendritic cell subsets, thus confounding the ability to identify these subsets following treatment. This has implications with regard to tracking the response to LPS in terms of the cell subsets involved, and should be considered whenever such studies are undertaken.

U2 - 10.1111/jcmm.12332

DO - 10.1111/jcmm.12332

M3 - Article

VL - 18

SP - 1908

EP - 1912

JO - Journal of Cellular and Molecular Medicine

JF - Journal of Cellular and Molecular Medicine

SN - 1582-1838

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