The identification and characterization of articular cartilage progenitor cell, which enabled the rapid culture of undifferentiated chondrocytes, were discussed. Chondrocytes were isolated from the surface, middle and deep zones of 7-day old bovine metacarpophalangeal joints by fine dissection and enzyme dissection. Also, colonies of chondrocytes consisting of more than 32 cells were counted at 10 days after differential adhesion and a colony forming efficiency (CFE) was also calculated. At 10 days, the CFE and colony size of surface zone chondrocytes initially cultured on fibronectin for 20 minutes was found to be greater than that of all other samples.
|Number of pages||1|
|Publication status||Published - 2002|
|Event||Third Smith and Nephew International Symposium - Translating Tissue Engineering into Products - Atlanta, United States|
Duration: 13 Oct 2002 → 16 Oct 2002
|Conference||Third Smith and Nephew International Symposium - Translating Tissue Engineering into Products|
|Period||13/10/02 → 16/10/02|