Characterisation of an articular cartilage progenitor cell

G. P. Dowthwaite; J. C. Bishop; S. Boyer; B. Thomson; M. Smith; C. W. Archer

Characterisation of an articular cartilage progenitor cell

G. P. Dowthwaite^*, J. C. Bishop, S. Boyer, B. Thomson, M. Smith, C. W. Archer

^*Corresponding author for this work

Research output: Contribution to conference › Abstract › Research › peer-review

Abstract

The identification and characterization of articular cartilage progenitor cell, which enabled the rapid culture of undifferentiated chondrocytes, were discussed. Chondrocytes were isolated from the surface, middle and deep zones of 7-day old bovine metacarpophalangeal joints by fine dissection and enzyme dissection. Also, colonies of chondrocytes consisting of more than 32 cells were counted at 10 days after differential adhesion and a colony forming efficiency (CFE) was also calculated. At 10 days, the CFE and colony size of surface zone chondrocytes initially cultured on fibronectin for 20 minutes was found to be greater than that of all other samples.

Original language	English
Pages	78
Number of pages	1
Publication status	Published - 2002
Externally published	Yes
Event	Third Smith and Nephew International Symposium - Translating Tissue Engineering into Products - Atlanta, United States Duration: 13 Oct 2002 → 16 Oct 2002

Conference

Conference	Third Smith and Nephew International Symposium - Translating Tissue Engineering into Products
Country/Territory	United States
City	Atlanta
Period	13/10/02 → 16/10/02

Cite this

@conference{ed24fb88d2624481a38a5fb25126c34e,

title = "Characterisation of an articular cartilage progenitor cell",

abstract = "The identification and characterization of articular cartilage progenitor cell, which enabled the rapid culture of undifferentiated chondrocytes, were discussed. Chondrocytes were isolated from the surface, middle and deep zones of 7-day old bovine metacarpophalangeal joints by fine dissection and enzyme dissection. Also, colonies of chondrocytes consisting of more than 32 cells were counted at 10 days after differential adhesion and a colony forming efficiency (CFE) was also calculated. At 10 days, the CFE and colony size of surface zone chondrocytes initially cultured on fibronectin for 20 minutes was found to be greater than that of all other samples.",

author = "Dowthwaite, {G. P.} and Bishop, {J. C.} and S. Boyer and B. Thomson and M. Smith and Archer, {C. W.}",

year = "2002",

language = "English",

pages = "78",

note = "Third Smith and Nephew International Symposium - Translating Tissue Engineering into Products ; Conference date: 13-10-2002 Through 16-10-2002",

}

TY - CONF

T1 - Characterisation of an articular cartilage progenitor cell

AU - Dowthwaite, G. P.

AU - Bishop, J. C.

AU - Boyer, S.

AU - Thomson, B.

AU - Smith, M.

AU - Archer, C. W.

PY - 2002

Y1 - 2002

N2 - The identification and characterization of articular cartilage progenitor cell, which enabled the rapid culture of undifferentiated chondrocytes, were discussed. Chondrocytes were isolated from the surface, middle and deep zones of 7-day old bovine metacarpophalangeal joints by fine dissection and enzyme dissection. Also, colonies of chondrocytes consisting of more than 32 cells were counted at 10 days after differential adhesion and a colony forming efficiency (CFE) was also calculated. At 10 days, the CFE and colony size of surface zone chondrocytes initially cultured on fibronectin for 20 minutes was found to be greater than that of all other samples.

AB - The identification and characterization of articular cartilage progenitor cell, which enabled the rapid culture of undifferentiated chondrocytes, were discussed. Chondrocytes were isolated from the surface, middle and deep zones of 7-day old bovine metacarpophalangeal joints by fine dissection and enzyme dissection. Also, colonies of chondrocytes consisting of more than 32 cells were counted at 10 days after differential adhesion and a colony forming efficiency (CFE) was also calculated. At 10 days, the CFE and colony size of surface zone chondrocytes initially cultured on fibronectin for 20 minutes was found to be greater than that of all other samples.

UR - http://www.scopus.com/inward/record.url?scp=18644382213&partnerID=8YFLogxK

M3 - Abstract

AN - SCOPUS:18644382213

SP - 78

T2 - Third Smith and Nephew International Symposium - Translating Tissue Engineering into Products

Y2 - 13 October 2002 through 16 October 2002

ER -

Characterisation of an articular cartilage progenitor cell

Abstract

Conference

Other files and links

Fingerprint

Cite this