Cell-cell contact and membrane spreading in an ultrasound trap

W T Coakley, D Bazou, J Morgan, G A Foster, C. W. Archer, K Powell, K A J Borthwick, C Twomey, Joanna Bishop

Research output: Contribution to journalArticleResearchpeer-review

34 Citations (Scopus)

Abstract

An ultrasonic standing wave trap [Langmuir 19 (2003) 3635] in which the morphologies of 2-D latex–microparticle aggregates, forming a pressure node plane, were characterised has been applied here to different cell suspensions with increasing order of specificity of cross-linking molecule, i.e. polylysine with chondrocytes; wheat germ agglutinin (WGA) with erythrocytes and surface receptors on neural cells. The outcome of initial cell–cell contact, i.e. whether the cells stuck at the point of contact (collision efficiency=1) or rolled around each other (collision efficiency=0), was monitored in situ by video-microscopy. The perimeter fractal dimensions (FD) of 2-D hexagonally symmetric, closely packed aggregates of control erythrocytes and chondrocytes were 1.16 and 1.18, respectively while those for the dendrititc aggregates formed initially by erythrocytes in 0.5 μg/ml WGA and chondrocytes in 20 μg/ml polylysine were 1.49 and 1.66. The FDs for control and molecularly cross-linked cells were typical of reaction-limited aggregation (RLA) and transport diffusion-limited aggregation (DLA), respectively. The FDs of the aggregates of cross-linked cells decreased with time to give more closely packed aggregates without clear hexagonal symmetry. Suspensions of neural cells formed dendritic aggregates. Spreading of inter-cellular membrane contact area occurred over 15 min for both erythrocyte and neural cell dendritic aggregates. The potential of the technique to characterise and control the progression of cell adhesion in suspension away from solid substrata is discussed.
Original languageEnglish
Article number209
Pages (from-to)221-230
JournalColloids and Surfaces B: Biointerfaces
Volume34
Issue number4
DOIs
Publication statusPublished - 15 Apr 2004
Externally publishedYes

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Ultrasonics
Cell Membrane
traps
membranes
Membranes
Chondrocytes
erythrocytes
Erythrocytes
cells
Suspensions
Wheat Germ Agglutinins
Polylysine
Dendritic Cells
wheat
Video Microscopy
Fractals
Agglomeration
Sensory Receptor Cells
Cell Adhesion
collisions

Cite this

Coakley, W. T., Bazou, D., Morgan, J., Foster, G. A., Archer, C. W., Powell, K., ... Bishop, J. (2004). Cell-cell contact and membrane spreading in an ultrasound trap. Colloids and Surfaces B: Biointerfaces, 34(4), 221-230. [209]. https://doi.org/10.1016/j.colsurfb.2004.01.002
Coakley, W T ; Bazou, D ; Morgan, J ; Foster, G A ; Archer, C. W. ; Powell, K ; Borthwick, K A J ; Twomey, C ; Bishop, Joanna . / Cell-cell contact and membrane spreading in an ultrasound trap. In: Colloids and Surfaces B: Biointerfaces. 2004 ; Vol. 34, No. 4. pp. 221-230.
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Coakley, WT, Bazou, D, Morgan, J, Foster, GA, Archer, CW, Powell, K, Borthwick, KAJ, Twomey, C & Bishop, J 2004, 'Cell-cell contact and membrane spreading in an ultrasound trap' Colloids and Surfaces B: Biointerfaces, vol. 34, no. 4, 209, pp. 221-230. https://doi.org/10.1016/j.colsurfb.2004.01.002

Cell-cell contact and membrane spreading in an ultrasound trap. / Coakley, W T ; Bazou, D; Morgan, J; Foster, G A; Archer, C. W.; Powell, K; Borthwick, K A J; Twomey, C; Bishop, Joanna .

In: Colloids and Surfaces B: Biointerfaces, Vol. 34, No. 4, 209, 15.04.2004, p. 221-230.

Research output: Contribution to journalArticleResearchpeer-review

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Coakley WT, Bazou D, Morgan J, Foster GA, Archer CW, Powell K et al. Cell-cell contact and membrane spreading in an ultrasound trap. Colloids and Surfaces B: Biointerfaces. 2004 Apr 15;34(4):221-230. 209. https://doi.org/10.1016/j.colsurfb.2004.01.002