Calcium phosphate stability on melt electrowritten PCL scaffolds

Naghmeh Abbasi; Stephen Hamlet; Van Thanh Dau; Nam Trung Nguyen

doi:10.1016/j.jsamd.2020.01.001

Calcium phosphate stability on melt electrowritten PCL scaffolds

Naghmeh Abbasi, Stephen Hamlet, Van Thanh Dau, Nam Trung Nguyen^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Research › peer-review

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Abstract

Calcium phosphate (CaP) coating on melt electrowritten (MEW) substrates is a potential candidate for bone regeneration influencing the interaction of osteoblasts with implanted scaffolds. Pretreatment to improve hydrophilicity of the hydrophobic polymer fibres affects subsequent coating with bioactive compounds like CaP. Therefore, this study evaluated the subsequent stability and structural properties of CaP coated MEW Poly-ε-caprolactone (PCL) scaffolds following pre-treatment with either argon-oxygen plasma or sodium hydroxide (NaOH). Scanning electron microscopy and μ-CT showed uniform CaP coating after one hour immersion in simulated body fluid following plasma pretreatment. Moreover, fourier transform infrared spectroscopy, energy dispersive spectrometry and X-ray diffraction analysis confirmed the presence of hydroxyapatite, tetracalcium phosphate and halite structures on the coated scaffolds. Contact angle measurement showed that the plasma pretreatment and CaP coating improved the hydrophilicity of the scaffold. However, the mechanical properties of the scaffolds were degraded after both plasma and NaOH treatments. The tensile stability was significantly improved following mineralization in plasma-treated scaffolds due to the smaller crystal size formed on the surface resulting in a dense CaP layer. The results obtained by thermogravimetric analysis also confirmed higher deposition of CaP particles on coated scaffolds following plasma modification. The results of this study show that plasma pre-treated mineralized MEW PCL scaffolds are sufficiently stable to be useful for further development in bone regeneration applications.

Original language	English
Pages (from-to)	30-39
Number of pages	10
Journal	Journal of Science: Advanced Materials and Devices
Volume	5
Issue number	1
DOIs	https://doi.org/10.1016/j.jsamd.2020.01.001
Publication status	Published - Mar 2020
Externally published	Yes

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Calcium phosphate stability on melt electrowritten PCL scaffoldsFinal published version, 2.42 MBLicence: CC BY

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@article{79bd631aead04ea6aa6e95427b99b8cd,

title = "Calcium phosphate stability on melt electrowritten PCL scaffolds",

abstract = "Calcium phosphate (CaP) coating on melt electrowritten (MEW) substrates is a potential candidate for bone regeneration influencing the interaction of osteoblasts with implanted scaffolds. Pretreatment to improve hydrophilicity of the hydrophobic polymer fibres affects subsequent coating with bioactive compounds like CaP. Therefore, this study evaluated the subsequent stability and structural properties of CaP coated MEW Poly-ε-caprolactone (PCL) scaffolds following pre-treatment with either argon-oxygen plasma or sodium hydroxide (NaOH). Scanning electron microscopy and μ-CT showed uniform CaP coating after one hour immersion in simulated body fluid following plasma pretreatment. Moreover, fourier transform infrared spectroscopy, energy dispersive spectrometry and X-ray diffraction analysis confirmed the presence of hydroxyapatite, tetracalcium phosphate and halite structures on the coated scaffolds. Contact angle measurement showed that the plasma pretreatment and CaP coating improved the hydrophilicity of the scaffold. However, the mechanical properties of the scaffolds were degraded after both plasma and NaOH treatments. The tensile stability was significantly improved following mineralization in plasma-treated scaffolds due to the smaller crystal size formed on the surface resulting in a dense CaP layer. The results obtained by thermogravimetric analysis also confirmed higher deposition of CaP particles on coated scaffolds following plasma modification. The results of this study show that plasma pre-treated mineralized MEW PCL scaffolds are sufficiently stable to be useful for further development in bone regeneration applications.",

author = "Naghmeh Abbasi and Stephen Hamlet and Dau, {Van Thanh} and Nguyen, {Nam Trung}",

note = "Funding Information: Naghmeh Abbasi was sponsored by a scholarship from Griffith University , Australia. This study is part of her PhD research project being carried out at Griffith University. The authors would like to express their gratitude to Institute of Health and Biomedical Innovation (IHBI) and the Central Analytical Research Facility (CARF) of QUT, Australia, for providing some facilities and technical support for this study, along with Dr. Abdalla Abdal-hay for his advice and general supervision of this research. We want to acknowledge and thank Alan White and Niloofar Ordou for their technical support. Publisher Copyright: {\textcopyright} 2020 The Authors Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",

year = "2020",

month = mar,

doi = "10.1016/j.jsamd.2020.01.001",

language = "English",

volume = "5",

pages = "30--39",

journal = "Journal of Science: Advanced Materials and Devices",

issn = "2468-2284",

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T1 - Calcium phosphate stability on melt electrowritten PCL scaffolds

AU - Abbasi, Naghmeh

AU - Hamlet, Stephen

AU - Dau, Van Thanh

AU - Nguyen, Nam Trung

N1 - Funding Information: Naghmeh Abbasi was sponsored by a scholarship from Griffith University , Australia. This study is part of her PhD research project being carried out at Griffith University. The authors would like to express their gratitude to Institute of Health and Biomedical Innovation (IHBI) and the Central Analytical Research Facility (CARF) of QUT, Australia, for providing some facilities and technical support for this study, along with Dr. Abdalla Abdal-hay for his advice and general supervision of this research. We want to acknowledge and thank Alan White and Niloofar Ordou for their technical support. Publisher Copyright: © 2020 The Authors Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

PY - 2020/3

Y1 - 2020/3

N2 - Calcium phosphate (CaP) coating on melt electrowritten (MEW) substrates is a potential candidate for bone regeneration influencing the interaction of osteoblasts with implanted scaffolds. Pretreatment to improve hydrophilicity of the hydrophobic polymer fibres affects subsequent coating with bioactive compounds like CaP. Therefore, this study evaluated the subsequent stability and structural properties of CaP coated MEW Poly-ε-caprolactone (PCL) scaffolds following pre-treatment with either argon-oxygen plasma or sodium hydroxide (NaOH). Scanning electron microscopy and μ-CT showed uniform CaP coating after one hour immersion in simulated body fluid following plasma pretreatment. Moreover, fourier transform infrared spectroscopy, energy dispersive spectrometry and X-ray diffraction analysis confirmed the presence of hydroxyapatite, tetracalcium phosphate and halite structures on the coated scaffolds. Contact angle measurement showed that the plasma pretreatment and CaP coating improved the hydrophilicity of the scaffold. However, the mechanical properties of the scaffolds were degraded after both plasma and NaOH treatments. The tensile stability was significantly improved following mineralization in plasma-treated scaffolds due to the smaller crystal size formed on the surface resulting in a dense CaP layer. The results obtained by thermogravimetric analysis also confirmed higher deposition of CaP particles on coated scaffolds following plasma modification. The results of this study show that plasma pre-treated mineralized MEW PCL scaffolds are sufficiently stable to be useful for further development in bone regeneration applications.

AB - Calcium phosphate (CaP) coating on melt electrowritten (MEW) substrates is a potential candidate for bone regeneration influencing the interaction of osteoblasts with implanted scaffolds. Pretreatment to improve hydrophilicity of the hydrophobic polymer fibres affects subsequent coating with bioactive compounds like CaP. Therefore, this study evaluated the subsequent stability and structural properties of CaP coated MEW Poly-ε-caprolactone (PCL) scaffolds following pre-treatment with either argon-oxygen plasma or sodium hydroxide (NaOH). Scanning electron microscopy and μ-CT showed uniform CaP coating after one hour immersion in simulated body fluid following plasma pretreatment. Moreover, fourier transform infrared spectroscopy, energy dispersive spectrometry and X-ray diffraction analysis confirmed the presence of hydroxyapatite, tetracalcium phosphate and halite structures on the coated scaffolds. Contact angle measurement showed that the plasma pretreatment and CaP coating improved the hydrophilicity of the scaffold. However, the mechanical properties of the scaffolds were degraded after both plasma and NaOH treatments. The tensile stability was significantly improved following mineralization in plasma-treated scaffolds due to the smaller crystal size formed on the surface resulting in a dense CaP layer. The results obtained by thermogravimetric analysis also confirmed higher deposition of CaP particles on coated scaffolds following plasma modification. The results of this study show that plasma pre-treated mineralized MEW PCL scaffolds are sufficiently stable to be useful for further development in bone regeneration applications.

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U2 - 10.1016/j.jsamd.2020.01.001

DO - 10.1016/j.jsamd.2020.01.001

M3 - Article

AN - SCOPUS:85078848009

SN - 2468-2284

VL - 5

SP - 30

EP - 39

JO - Journal of Science: Advanced Materials and Devices

JF - Journal of Science: Advanced Materials and Devices

IS - 1

ER -

Calcium phosphate stability on melt electrowritten PCL scaffolds

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