Abstract
A perfect bone implant must be made of porous gradient biomaterials which mimic the properties of natural bone ECM with porosity-graded structure (1). Proper architecture of porous scaffold provides an environment for higher cell infiltration and promotes nutrient fluids to the structure while it has potential to withstand external loading stress. The previous studies
displayed more cell growth and nutrient-waste flow transport in highly porous region since of its greater surface area, while the dense layer proved that is favorable to external loading of
mechanical need (2).
In this study, we designed three-dimensional electrospun melt electrospun e-poly caprolactone (PCL) scaffolds with different pore size and various offset values (30%, 50%) and with gradually increasing pore size along the longitudinal direction (from 250 to750 µm), a square pore shape which have been fabricated by melt electrospinning writing technique. Surface modification of scaffold with bioactive materials has been used throughout the scaffold which has impressive effect to promote cell attachment and proliferation.
The fabricated scaffolds were estimated for physical properties, including electron microscopy, water-uptake ability, and calculated bending modulus. In addition, they were also evaluated for biological capabilities by culturing osteoblast cells for various structure of the scaffolds. Compared with the simple structures, the gradient and offset scaffolds showed superior physical and biological improvements, such as higher bending modulus. Cell-seeding efficiency and cell viability were assessed using cell imaging and DNA assay that showed better attachment and growth in gradient structure.
The pore size gradient scaffolds can be a good candidate for the bone formation studies.
References:
1. Karageorgiou V, Kaplan D. Porosity of 3D biomateria! scaffolds and osteogenesis. Blomaterials 2005;26:5474-91.
2. Yeo MG, Simon Jr CG, Kim GH. Effects of offset values of solid freeform fabricated PCL-b·TCP scaffolds on
mechanical proper-ties and cellular activities in bone tissue regeneration. J Mater Chem 2012;22:21636-
21646.
displayed more cell growth and nutrient-waste flow transport in highly porous region since of its greater surface area, while the dense layer proved that is favorable to external loading of
mechanical need (2).
In this study, we designed three-dimensional electrospun melt electrospun e-poly caprolactone (PCL) scaffolds with different pore size and various offset values (30%, 50%) and with gradually increasing pore size along the longitudinal direction (from 250 to750 µm), a square pore shape which have been fabricated by melt electrospinning writing technique. Surface modification of scaffold with bioactive materials has been used throughout the scaffold which has impressive effect to promote cell attachment and proliferation.
The fabricated scaffolds were estimated for physical properties, including electron microscopy, water-uptake ability, and calculated bending modulus. In addition, they were also evaluated for biological capabilities by culturing osteoblast cells for various structure of the scaffolds. Compared with the simple structures, the gradient and offset scaffolds showed superior physical and biological improvements, such as higher bending modulus. Cell-seeding efficiency and cell viability were assessed using cell imaging and DNA assay that showed better attachment and growth in gradient structure.
The pore size gradient scaffolds can be a good candidate for the bone formation studies.
References:
1. Karageorgiou V, Kaplan D. Porosity of 3D biomateria! scaffolds and osteogenesis. Blomaterials 2005;26:5474-91.
2. Yeo MG, Simon Jr CG, Kim GH. Effects of offset values of solid freeform fabricated PCL-b·TCP scaffolds on
mechanical proper-ties and cellular activities in bone tissue regeneration. J Mater Chem 2012;22:21636-
21646.
| Original language | English |
|---|---|
| Publication status | Unpublished - 8 Jun 2017 |
| Externally published | Yes |
| Event | School of Dentistry & Oral Health Research Day - Griffith Universtiy, Australia Duration: 8 Jun 2017 → 8 Jun 2017 |
Conference
| Conference | School of Dentistry & Oral Health Research Day |
|---|---|
| Abbreviated title | DOH |
| Country/Territory | Australia |
| Period | 8/06/17 → 8/06/17 |