Biomimetic poly(lactide) based fibrous scaffolds for ligament tissue engineering

Denver C Surrao, Stephen D Waldman, Brian G Amsden

Research output: Contribution to journalArticleResearchpeer-review

38 Citations (Scopus)

Abstract

The aim of this study was to fabricate a fibrous scaffold that closely resembled the micro-structural architecture and mechanical properties of collagen fibres found in the anterior cruciate ligament (ACL). To achieve this aim, fibrous scaffolds were made by electrospinning L-lactide based polymers. L-Lactide was chosen primarily due to its demonstrated biocompatibility, biodegradability and high modulus. The electrospun fibres were collected in tension on a rotating wire mandrel. Upon treating these fibres in a heated aqueous environment, they possessed a crimp-like pattern having a wavelength and amplitude similar to that of native ACL collagen. Of the polymer fibre scaffolds studied, those made from poly(L-lactide-co-D,L-lactide) PLDLA exhibited the highest modulus and were also the most resilient to in vitro hydrolytic degradation, undergoing a slight decrease in modulus compared to the other polymeric fibres over a 6 month period. Bovine fibroblasts seeded on the wavy, crimp-like PLDLA fibres attached, proliferated and deposited extracellular matrix (ECM) molecules on the surface of the fibrous scaffold. In addition, the deposited ECM exhibited bundle formation that resembled the fascicles found in native ACL. These findings demonstrate the importance of replicating the geometric microenvironment in developing effective tissue engineering scaffolds.

Original languageEnglish
Pages (from-to)3997-4006
Number of pages10
JournalActa Biomaterialia
Volume8
Issue number11
DOIs
Publication statusPublished - Nov 2012
Externally publishedYes

Fingerprint

Biomimetics
Anterior Cruciate Ligament
Ligaments
Tissue Engineering
Scaffolds (biology)
Tissue engineering
Scaffolds
Extracellular Matrix
Fibers
Polymers
Collagen
Tissue Scaffolds
Fibroblasts
Biodegradability
Electrospinning
Biocompatibility
poly(lactide)
Wire
Degradation
Wavelength

Cite this

Surrao, Denver C ; Waldman, Stephen D ; Amsden, Brian G. / Biomimetic poly(lactide) based fibrous scaffolds for ligament tissue engineering. In: Acta Biomaterialia. 2012 ; Vol. 8, No. 11. pp. 3997-4006.
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abstract = "The aim of this study was to fabricate a fibrous scaffold that closely resembled the micro-structural architecture and mechanical properties of collagen fibres found in the anterior cruciate ligament (ACL). To achieve this aim, fibrous scaffolds were made by electrospinning L-lactide based polymers. L-Lactide was chosen primarily due to its demonstrated biocompatibility, biodegradability and high modulus. The electrospun fibres were collected in tension on a rotating wire mandrel. Upon treating these fibres in a heated aqueous environment, they possessed a crimp-like pattern having a wavelength and amplitude similar to that of native ACL collagen. Of the polymer fibre scaffolds studied, those made from poly(L-lactide-co-D,L-lactide) PLDLA exhibited the highest modulus and were also the most resilient to in vitro hydrolytic degradation, undergoing a slight decrease in modulus compared to the other polymeric fibres over a 6 month period. Bovine fibroblasts seeded on the wavy, crimp-like PLDLA fibres attached, proliferated and deposited extracellular matrix (ECM) molecules on the surface of the fibrous scaffold. In addition, the deposited ECM exhibited bundle formation that resembled the fascicles found in native ACL. These findings demonstrate the importance of replicating the geometric microenvironment in developing effective tissue engineering scaffolds.",
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Biomimetic poly(lactide) based fibrous scaffolds for ligament tissue engineering. / Surrao, Denver C; Waldman, Stephen D; Amsden, Brian G.

In: Acta Biomaterialia, Vol. 8, No. 11, 11.2012, p. 3997-4006.

Research output: Contribution to journalArticleResearchpeer-review

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