Automatic alignment and comparison on images of petri dishes containing cell colonies

Safar Alqahtani, Andre Barczak, Napoleon Reyes, Teo Susnjak, Austen Ganley

Research output: Chapter in Book/Report/Conference proceedingConference contributionResearchpeer-review

Abstract

This paper proposes a novel approach to comparing cell colony images taken at different times on a Petri dish. The objective is to provide an assistive tool for microbiologists to quantify the loss of cell colonies on two Petri dishes, by mapping cell colonies between a pair of images. This problem is highly non-trivial, as the shape, size and position of the corresponding colonies vary randomly. In addition, the cell colony images for comparison are taken at different times and from slightly different perspectives (i.e. effects of shearing), and this amplifies the complexity of the problem. Experiments show that approaches purely based on SIFT or SURF, or algorithms used in astronomy, do not perform well on the problem domain. We therefore introduce a new approach to addressing these problems. A novel iterative technique that combines triangulation algorithms with the RANSAC alignment algorithm is proposed. Through hundreds of experiments, we demonstrate the efficacy of the new algorithm in comparison to existing ones found in the literature.

Original languageEnglish
Title of host publication2015 International Conference on Image and Vision Computing New Zealand, IVCNZ 2015
PublisherIEEE Computer Society
ISBN (Electronic)9781509003570
DOIs
Publication statusPublished - 28 Nov 2016
Externally publishedYes
Event2015 International Conference on Image and Vision Computing New Zealand, IVCNZ 2015 - Auckland, New Zealand
Duration: 23 Nov 201524 Nov 2015

Publication series

NameInternational Conference Image and Vision Computing New Zealand
Volume2016-November
ISSN (Print)2151-2191
ISSN (Electronic)2151-2205

Conference

Conference2015 International Conference on Image and Vision Computing New Zealand, IVCNZ 2015
Abbreviated titleIVCNZ
Country/TerritoryNew Zealand
CityAuckland
Period23/11/1524/11/15

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