Assessment of immune function after short-term administration of recombinant human growth hormone in healthy young males

S. B. Ramos, E. W. Brenu, R. Christy, B. Gray, L. McNaughton, L. Tajouri, M. Van Driel, S. M. Marshall-Gradisnik

Research output: Contribution to journalArticleResearchpeer-review

3 Citations (Scopus)

Abstract

Growth hormone (GH) is a commonly used drug aimed at improving sport performance. The aim of this study is to evaluate the immunomodulatory effects of short-term administration of recombinant GH (rhGH) in healthy young males. NK cell number, activity and phenotype, T cell number, CD4+ (Th1/Th2) cytokine production of IL2, IL4, IL6, IL10, TNF-α and IFN-γ and CD4+/CD8+ ratio with particular attention to the possible correlation to IGF-I production were investigated. 30 males (27 ± 9 years) were randomly assigned to placebo (n = 15) or drug (rhGH) 1 mg/day groups data were generated at baseline and days 8, 15, 22 and 29 post injection. Data analysis used General Linear Model with repeated measures, Bonferroni correction factor and signiWcance at p ≤ 0.05. Serum IGF-I levels (ng/mL) increased signiWcantly (p ≤ 0.01) on day 8 (0.48 ± 0.78) after injections compared to baseline (0.31 ± 0.07) and days 15 (0.33 ± 0.06), 22 (0.29 ± 0.05) and 29 (.29 ± 0.06). A signiWcant time eVect was noted in IL10 secretion (pg/mL) from day 15 (P = 35.14 ± 19.93, rhGH = 26.63 ± 16.39) to days 22 (P = 61.32 ± 20.41, rhGH = 74.99 ± 46.91) and 29 (P = 101.98 ± 67.25, rhGH = 107.74; ± 122.58). There was no correlation between IGF-I and NK activity, phenotype or number along with Tlymphocyte number, CD4+/CD8+ ratio or Th1 and Th2 cytokine production. In conclusion, cytokine secretion spectrum was not aVected by short-term rhGH administration in young males

Original languageEnglish
Pages (from-to)1307-1312
Number of pages6
JournalEuropean Journal of Applied Physiology
Volume111
Issue number7
Early online date8 Dec 2010
DOIs
Publication statusPublished - Jul 2011

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Human Growth Hormone
Insulin-Like Growth Factor I
Growth Hormone
CD4-CD8 Ratio
Cytokines
Interleukin-10
Cell Count
Phenotype
Athletic Performance
Injections
Natural Killer Cells
Interleukin-4
Pharmaceutical Preparations
Interleukin-2
Linear Models
Interleukin-6
Placebos
T-Lymphocytes
Serum

Cite this

Ramos, S. B. ; Brenu, E. W. ; Christy, R. ; Gray, B. ; McNaughton, L. ; Tajouri, L. ; Van Driel, M. ; Marshall-Gradisnik, S. M. / Assessment of immune function after short-term administration of recombinant human growth hormone in healthy young males. In: European Journal of Applied Physiology. 2011 ; Vol. 111, No. 7. pp. 1307-1312.
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title = "Assessment of immune function after short-term administration of recombinant human growth hormone in healthy young males",
abstract = "Growth hormone (GH) is a commonly used drug aimed at improving sport performance. The aim of this study is to evaluate the immunomodulatory effects of short-term administration of recombinant GH (rhGH) in healthy young males. NK cell number, activity and phenotype, T cell number, CD4+ (Th1/Th2) cytokine production of IL2, IL4, IL6, IL10, TNF-α and IFN-γ and CD4+/CD8+ ratio with particular attention to the possible correlation to IGF-I production were investigated. 30 males (27 ± 9 years) were randomly assigned to placebo (n = 15) or drug (rhGH) 1 mg/day groups data were generated at baseline and days 8, 15, 22 and 29 post injection. Data analysis used General Linear Model with repeated measures, Bonferroni correction factor and signiWcance at p ≤ 0.05. Serum IGF-I levels (ng/mL) increased signiWcantly (p ≤ 0.01) on day 8 (0.48 ± 0.78) after injections compared to baseline (0.31 ± 0.07) and days 15 (0.33 ± 0.06), 22 (0.29 ± 0.05) and 29 (.29 ± 0.06). A signiWcant time eVect was noted in IL10 secretion (pg/mL) from day 15 (P = 35.14 ± 19.93, rhGH = 26.63 ± 16.39) to days 22 (P = 61.32 ± 20.41, rhGH = 74.99 ± 46.91) and 29 (P = 101.98 ± 67.25, rhGH = 107.74; ± 122.58). There was no correlation between IGF-I and NK activity, phenotype or number along with Tlymphocyte number, CD4+/CD8+ ratio or Th1 and Th2 cytokine production. In conclusion, cytokine secretion spectrum was not aVected by short-term rhGH administration in young males",
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Assessment of immune function after short-term administration of recombinant human growth hormone in healthy young males. / Ramos, S. B.; Brenu, E. W.; Christy, R.; Gray, B.; McNaughton, L.; Tajouri, L.; Van Driel, M.; Marshall-Gradisnik, S. M.

In: European Journal of Applied Physiology, Vol. 111, No. 7, 07.2011, p. 1307-1312.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Ramos, S. B.

AU - Brenu, E. W.

AU - Christy, R.

AU - Gray, B.

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AU - Marshall-Gradisnik, S. M.

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AB - Growth hormone (GH) is a commonly used drug aimed at improving sport performance. The aim of this study is to evaluate the immunomodulatory effects of short-term administration of recombinant GH (rhGH) in healthy young males. NK cell number, activity and phenotype, T cell number, CD4+ (Th1/Th2) cytokine production of IL2, IL4, IL6, IL10, TNF-α and IFN-γ and CD4+/CD8+ ratio with particular attention to the possible correlation to IGF-I production were investigated. 30 males (27 ± 9 years) were randomly assigned to placebo (n = 15) or drug (rhGH) 1 mg/day groups data were generated at baseline and days 8, 15, 22 and 29 post injection. Data analysis used General Linear Model with repeated measures, Bonferroni correction factor and signiWcance at p ≤ 0.05. Serum IGF-I levels (ng/mL) increased signiWcantly (p ≤ 0.01) on day 8 (0.48 ± 0.78) after injections compared to baseline (0.31 ± 0.07) and days 15 (0.33 ± 0.06), 22 (0.29 ± 0.05) and 29 (.29 ± 0.06). A signiWcant time eVect was noted in IL10 secretion (pg/mL) from day 15 (P = 35.14 ± 19.93, rhGH = 26.63 ± 16.39) to days 22 (P = 61.32 ± 20.41, rhGH = 74.99 ± 46.91) and 29 (P = 101.98 ± 67.25, rhGH = 107.74; ± 122.58). There was no correlation between IGF-I and NK activity, phenotype or number along with Tlymphocyte number, CD4+/CD8+ ratio or Th1 and Th2 cytokine production. In conclusion, cytokine secretion spectrum was not aVected by short-term rhGH administration in young males

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