Workplace stress is common across occupations and across nations. However, there has been limited research examining the similarities and differences across cultures, and none that seem to have used a direct comparison across one professional area using the same extensive and validated questionnaire. One such questionnaire is the Occupational Stress Inventory-Revised (OSI-R: Osipow 1998) which assesses three main dimensions related to stress: "occupational roles" (stressors), "personal strain" (experienced stress), and "personal resources" (coping resources). The current study examined a cross-national application of the OSI-R among Australian and Turkish teachers to identify whether patterns of latent structure of the OSI-R were similar (and therefore whether the questionnaire would be useful in wider research and professional application). Structural equation modeling and fit indices results generally confirmed the three-dimensional model posited by Osipow and also suggested that the occupational role stress dimension could be sub-divided: the similar latent factor results obtained across cultures for the teacher samples suggesting the stability of the OSI-R in this cross-cultural workplace setting. The general implications for research and practice are discussed.