Articular Chondroprogenitors as tools for cartilage tissue engineering

Joanna Charlotte Bishop, I. M. Khan, R Williams, Charles Archer

Research output: Contribution to journalMeeting AbstractResearchpeer-review

Abstract

The use of stem/progenitor cells to form good quality cartilage matrix has been targeted as a therapeutic goal for cartilage repair treatment. We have characterised a progenitor cell population that resides in the surface layer of neonatal bovine articular cartilage [1]. Here, we assess the progenitor cell's chondrogenic potential. Cloned chondroprogenitors exhibited exponential growth for the first 20 population doublings (PD), then slower linear growth with evidence of replicative senescence at later passages. Mean telomere lengths of exponentially growing chondroprogenitors were significantly longer than dedifferentiated chondrocytes that had undergone a similar number of PD (P<0.05). Chondroprogenitors also had 2.6-fold greater telomerase activity and maintained sox9 and Notch-1 mRNA expression whereas dedifferentiated chondrocytes demonstrated little or no detectable expression. Induced differentiation to cartilage in 3D pellet cultures, demonstrated sox9, Notch-1, aggrecan and PCNA expression.Maintenenace of telomerase activity, Notch-1 and sox9 gene expression distinguish clonal chondroprogenitor cells from dedifferentiated chondrocytes. When placed in chondrogenic medium chondroprogenitors appear to engage in co-ordinated growth and differentiation in stark contrast to dedifferentiated chondrocytes. UK DBERF
Original languageEnglish
JournalFASEB Journal
Volume23
Issue number1 supplement
Publication statusPublished - 1 Apr 2009
Externally publishedYes
EventExperimental Biology 2009 - Ernest N. Morial Convention Center, New Orleans, United States
Duration: 18 Apr 200922 Apr 2009
https://www.aspet.org/aspet/meetings-awards/meetingsannual-meeting/annual-meeting-archives

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Cartilage
Tissue Engineering
Chondrocytes
Tissue engineering
Stem Cells
Joints
Telomerase
Growth
Population
Aggrecans
Cell Aging
Telomere
Proliferating Cell Nuclear Antigen
Articular Cartilage
Stem cells
Gene expression
Repair
Cells
Gene Expression
Messenger RNA

Cite this

Bishop, J. C., Khan, I. M., Williams, R., & Archer, C. (2009). Articular Chondroprogenitors as tools for cartilage tissue engineering. FASEB Journal, 23(1 supplement).
Bishop, Joanna Charlotte ; Khan, I. M. ; Williams, R ; Archer, Charles. / Articular Chondroprogenitors as tools for cartilage tissue engineering. In: FASEB Journal. 2009 ; Vol. 23, No. 1 supplement.
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Bishop, JC, Khan, IM, Williams, R & Archer, C 2009, 'Articular Chondroprogenitors as tools for cartilage tissue engineering' FASEB Journal, vol. 23, no. 1 supplement.

Articular Chondroprogenitors as tools for cartilage tissue engineering. / Bishop, Joanna Charlotte; Khan, I. M.; Williams, R; Archer, Charles.

In: FASEB Journal, Vol. 23, No. 1 supplement, 01.04.2009.

Research output: Contribution to journalMeeting AbstractResearchpeer-review

TY - JOUR

T1 - Articular Chondroprogenitors as tools for cartilage tissue engineering

AU - Bishop, Joanna Charlotte

AU - Khan, I. M.

AU - Williams, R

AU - Archer, Charles

PY - 2009/4/1

Y1 - 2009/4/1

N2 - The use of stem/progenitor cells to form good quality cartilage matrix has been targeted as a therapeutic goal for cartilage repair treatment. We have characterised a progenitor cell population that resides in the surface layer of neonatal bovine articular cartilage [1]. Here, we assess the progenitor cell's chondrogenic potential. Cloned chondroprogenitors exhibited exponential growth for the first 20 population doublings (PD), then slower linear growth with evidence of replicative senescence at later passages. Mean telomere lengths of exponentially growing chondroprogenitors were significantly longer than dedifferentiated chondrocytes that had undergone a similar number of PD (P<0.05). Chondroprogenitors also had 2.6-fold greater telomerase activity and maintained sox9 and Notch-1 mRNA expression whereas dedifferentiated chondrocytes demonstrated little or no detectable expression. Induced differentiation to cartilage in 3D pellet cultures, demonstrated sox9, Notch-1, aggrecan and PCNA expression.Maintenenace of telomerase activity, Notch-1 and sox9 gene expression distinguish clonal chondroprogenitor cells from dedifferentiated chondrocytes. When placed in chondrogenic medium chondroprogenitors appear to engage in co-ordinated growth and differentiation in stark contrast to dedifferentiated chondrocytes. UK DBERF

AB - The use of stem/progenitor cells to form good quality cartilage matrix has been targeted as a therapeutic goal for cartilage repair treatment. We have characterised a progenitor cell population that resides in the surface layer of neonatal bovine articular cartilage [1]. Here, we assess the progenitor cell's chondrogenic potential. Cloned chondroprogenitors exhibited exponential growth for the first 20 population doublings (PD), then slower linear growth with evidence of replicative senescence at later passages. Mean telomere lengths of exponentially growing chondroprogenitors were significantly longer than dedifferentiated chondrocytes that had undergone a similar number of PD (P<0.05). Chondroprogenitors also had 2.6-fold greater telomerase activity and maintained sox9 and Notch-1 mRNA expression whereas dedifferentiated chondrocytes demonstrated little or no detectable expression. Induced differentiation to cartilage in 3D pellet cultures, demonstrated sox9, Notch-1, aggrecan and PCNA expression.Maintenenace of telomerase activity, Notch-1 and sox9 gene expression distinguish clonal chondroprogenitor cells from dedifferentiated chondrocytes. When placed in chondrogenic medium chondroprogenitors appear to engage in co-ordinated growth and differentiation in stark contrast to dedifferentiated chondrocytes. UK DBERF

M3 - Meeting Abstract

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Bishop JC, Khan IM, Williams R, Archer C. Articular Chondroprogenitors as tools for cartilage tissue engineering. FASEB Journal. 2009 Apr 1;23(1 supplement).