Antibody which defines a subset of bone marrow cells that can migrate to thymus

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Abstract

A T-cell line of immature phenotype, 16C1, has been shown to have specific capacity to migrate to and localize in the thymus of irradiated mice. Several antibodies specific for the Pgp-1 molecule have been tested for their capacity to inhibit the migration of 16C1 and of bone marrow to thymus in just a 3-hr double-label migration assay. Analysis of Pgp-1 expression has revealed that 16C1, derived from C57BL mice, characteristically binds antibodies specific for the Pgp-1.1 and not the Pgp-1.2 allelic determinant, which is normally expressed by cells from C57BL mice. This unusual epitope expression has also been demonstrated for several other cell lines of similar origin to 16C1. A 5% subpopulation of C57BL bone marrow has also been defined which binds both the allele-specific antibodies. These cells exist amongst the class I+, Thy-1+, T200+ subpopulation of bone marrow. Anti-Pgp-1 antibodies have been shown to inhibit the thymus-homing capacity of both 16C1 and of bone marrow and have been used to deplete bone marrow of cells capable of reconstituting the T-cell compartment of irradiated mice. All of this indicates at least two distinct Pgp-1 determinants can be expressed by cells from C57BL mice and that antibody specific for either of these determinants can inhibit thymus homing capacity. T-cell precursors which can migrate to thymus would appear to exist amongst the subset of bone marrow cells which express both the Pgp-1.1 and Pgp-1.2 determinants.

Original languageEnglish
Pages (from-to)59-65
Number of pages7
JournalImmunology
Volume68
Issue number1
Publication statusPublished - Sep 1989
Externally publishedYes

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Bone Marrow Cells
Thymus Gland
Bone Marrow
Inbred C57BL Mouse
Antibodies
T-Lymphoid Precursor Cells
T-Lymphocytes
Cell Line
Epitopes
Alleles
Phenotype

Cite this

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title = "Antibody which defines a subset of bone marrow cells that can migrate to thymus",
abstract = "A T-cell line of immature phenotype, 16C1, has been shown to have specific capacity to migrate to and localize in the thymus of irradiated mice. Several antibodies specific for the Pgp-1 molecule have been tested for their capacity to inhibit the migration of 16C1 and of bone marrow to thymus in just a 3-hr double-label migration assay. Analysis of Pgp-1 expression has revealed that 16C1, derived from C57BL mice, characteristically binds antibodies specific for the Pgp-1.1 and not the Pgp-1.2 allelic determinant, which is normally expressed by cells from C57BL mice. This unusual epitope expression has also been demonstrated for several other cell lines of similar origin to 16C1. A 5{\%} subpopulation of C57BL bone marrow has also been defined which binds both the allele-specific antibodies. These cells exist amongst the class I+, Thy-1+, T200+ subpopulation of bone marrow. Anti-Pgp-1 antibodies have been shown to inhibit the thymus-homing capacity of both 16C1 and of bone marrow and have been used to deplete bone marrow of cells capable of reconstituting the T-cell compartment of irradiated mice. All of this indicates at least two distinct Pgp-1 determinants can be expressed by cells from C57BL mice and that antibody specific for either of these determinants can inhibit thymus homing capacity. T-cell precursors which can migrate to thymus would appear to exist amongst the subset of bone marrow cells which express both the Pgp-1.1 and Pgp-1.2 determinants.",
author = "O'Neill, {H C}",
year = "1989",
month = "9",
language = "English",
volume = "68",
pages = "59--65",
journal = "Immunology",
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}

Antibody which defines a subset of bone marrow cells that can migrate to thymus. / O'Neill, H C.

In: Immunology, Vol. 68, No. 1, 09.1989, p. 59-65.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Antibody which defines a subset of bone marrow cells that can migrate to thymus

AU - O'Neill, H C

PY - 1989/9

Y1 - 1989/9

N2 - A T-cell line of immature phenotype, 16C1, has been shown to have specific capacity to migrate to and localize in the thymus of irradiated mice. Several antibodies specific for the Pgp-1 molecule have been tested for their capacity to inhibit the migration of 16C1 and of bone marrow to thymus in just a 3-hr double-label migration assay. Analysis of Pgp-1 expression has revealed that 16C1, derived from C57BL mice, characteristically binds antibodies specific for the Pgp-1.1 and not the Pgp-1.2 allelic determinant, which is normally expressed by cells from C57BL mice. This unusual epitope expression has also been demonstrated for several other cell lines of similar origin to 16C1. A 5% subpopulation of C57BL bone marrow has also been defined which binds both the allele-specific antibodies. These cells exist amongst the class I+, Thy-1+, T200+ subpopulation of bone marrow. Anti-Pgp-1 antibodies have been shown to inhibit the thymus-homing capacity of both 16C1 and of bone marrow and have been used to deplete bone marrow of cells capable of reconstituting the T-cell compartment of irradiated mice. All of this indicates at least two distinct Pgp-1 determinants can be expressed by cells from C57BL mice and that antibody specific for either of these determinants can inhibit thymus homing capacity. T-cell precursors which can migrate to thymus would appear to exist amongst the subset of bone marrow cells which express both the Pgp-1.1 and Pgp-1.2 determinants.

AB - A T-cell line of immature phenotype, 16C1, has been shown to have specific capacity to migrate to and localize in the thymus of irradiated mice. Several antibodies specific for the Pgp-1 molecule have been tested for their capacity to inhibit the migration of 16C1 and of bone marrow to thymus in just a 3-hr double-label migration assay. Analysis of Pgp-1 expression has revealed that 16C1, derived from C57BL mice, characteristically binds antibodies specific for the Pgp-1.1 and not the Pgp-1.2 allelic determinant, which is normally expressed by cells from C57BL mice. This unusual epitope expression has also been demonstrated for several other cell lines of similar origin to 16C1. A 5% subpopulation of C57BL bone marrow has also been defined which binds both the allele-specific antibodies. These cells exist amongst the class I+, Thy-1+, T200+ subpopulation of bone marrow. Anti-Pgp-1 antibodies have been shown to inhibit the thymus-homing capacity of both 16C1 and of bone marrow and have been used to deplete bone marrow of cells capable of reconstituting the T-cell compartment of irradiated mice. All of this indicates at least two distinct Pgp-1 determinants can be expressed by cells from C57BL mice and that antibody specific for either of these determinants can inhibit thymus homing capacity. T-cell precursors which can migrate to thymus would appear to exist amongst the subset of bone marrow cells which express both the Pgp-1.1 and Pgp-1.2 determinants.

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