Alternate splicing and expression of the glutamate transporter EAAT5 in the rat retina

Aven Lee, Ashley R. Anderson, Nigel L. Barnett, Melissa G. Stevens, David V. Pow

Research output: Contribution to journalArticleResearchpeer-review

11 Citations (Scopus)

Abstract

Excitatory amino acid transporter 5 (EAAT5) is an unusual glutamate transporter that is expressed in the retina, where it is localised to two populations of glutamatergic neurons, namely the bipolar neurons and photoreceptors. EAAT5 exhibits two distinct properties, acting both as a slow glutamate transporter and as a glutamate-gated inhibitory receptor. The latter property is attributable to a co-associated chloride conductance. EAAT5 has previously been thought to exist only as a full-length form. We now demonstrate by PCR cloning and sequencing, the presence of five novel splice variant forms of EAAT5 which skip either partial or complete exons in the rat retina. Furthermore, we demonstrate that each of these variants is expressed at the protein level as assessed by Western blotting using splice-specific antibodies that we have generated. We conclude that EAAT5 exists in multiple spliced forms, and propose, based upon retention or absence of key structural features, that these variant forms may potentially exhibit distinct properties relative to the originally described form of EAAT5.

Original languageEnglish
Pages (from-to)283-288
Number of pages6
JournalGene
Volume506
Issue number2
DOIs
Publication statusPublished - 15 Sep 2012
Externally publishedYes

Fingerprint

Excitatory Amino Acid Transporter 5
Amino Acid Transport System X-AG
Alternative Splicing
Retina
Neurons
Organism Cloning
Chlorides
Glutamic Acid
Exons
Western Blotting
Polymerase Chain Reaction

Cite this

Lee, Aven ; Anderson, Ashley R. ; Barnett, Nigel L. ; Stevens, Melissa G. ; Pow, David V. / Alternate splicing and expression of the glutamate transporter EAAT5 in the rat retina. In: Gene. 2012 ; Vol. 506, No. 2. pp. 283-288.
@article{a96280ea4f4a42b6af881c3d557bca7e,
title = "Alternate splicing and expression of the glutamate transporter EAAT5 in the rat retina",
abstract = "Excitatory amino acid transporter 5 (EAAT5) is an unusual glutamate transporter that is expressed in the retina, where it is localised to two populations of glutamatergic neurons, namely the bipolar neurons and photoreceptors. EAAT5 exhibits two distinct properties, acting both as a slow glutamate transporter and as a glutamate-gated inhibitory receptor. The latter property is attributable to a co-associated chloride conductance. EAAT5 has previously been thought to exist only as a full-length form. We now demonstrate by PCR cloning and sequencing, the presence of five novel splice variant forms of EAAT5 which skip either partial or complete exons in the rat retina. Furthermore, we demonstrate that each of these variants is expressed at the protein level as assessed by Western blotting using splice-specific antibodies that we have generated. We conclude that EAAT5 exists in multiple spliced forms, and propose, based upon retention or absence of key structural features, that these variant forms may potentially exhibit distinct properties relative to the originally described form of EAAT5.",
author = "Aven Lee and Anderson, {Ashley R.} and Barnett, {Nigel L.} and Stevens, {Melissa G.} and Pow, {David V.}",
year = "2012",
month = "9",
day = "15",
doi = "10.1016/j.gene.2012.07.010",
language = "English",
volume = "506",
pages = "283--288",
journal = "Gene",
issn = "0378-1119",
publisher = "Elsevier",
number = "2",

}

Alternate splicing and expression of the glutamate transporter EAAT5 in the rat retina. / Lee, Aven; Anderson, Ashley R.; Barnett, Nigel L.; Stevens, Melissa G.; Pow, David V.

In: Gene, Vol. 506, No. 2, 15.09.2012, p. 283-288.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Alternate splicing and expression of the glutamate transporter EAAT5 in the rat retina

AU - Lee, Aven

AU - Anderson, Ashley R.

AU - Barnett, Nigel L.

AU - Stevens, Melissa G.

AU - Pow, David V.

PY - 2012/9/15

Y1 - 2012/9/15

N2 - Excitatory amino acid transporter 5 (EAAT5) is an unusual glutamate transporter that is expressed in the retina, where it is localised to two populations of glutamatergic neurons, namely the bipolar neurons and photoreceptors. EAAT5 exhibits two distinct properties, acting both as a slow glutamate transporter and as a glutamate-gated inhibitory receptor. The latter property is attributable to a co-associated chloride conductance. EAAT5 has previously been thought to exist only as a full-length form. We now demonstrate by PCR cloning and sequencing, the presence of five novel splice variant forms of EAAT5 which skip either partial or complete exons in the rat retina. Furthermore, we demonstrate that each of these variants is expressed at the protein level as assessed by Western blotting using splice-specific antibodies that we have generated. We conclude that EAAT5 exists in multiple spliced forms, and propose, based upon retention or absence of key structural features, that these variant forms may potentially exhibit distinct properties relative to the originally described form of EAAT5.

AB - Excitatory amino acid transporter 5 (EAAT5) is an unusual glutamate transporter that is expressed in the retina, where it is localised to two populations of glutamatergic neurons, namely the bipolar neurons and photoreceptors. EAAT5 exhibits two distinct properties, acting both as a slow glutamate transporter and as a glutamate-gated inhibitory receptor. The latter property is attributable to a co-associated chloride conductance. EAAT5 has previously been thought to exist only as a full-length form. We now demonstrate by PCR cloning and sequencing, the presence of five novel splice variant forms of EAAT5 which skip either partial or complete exons in the rat retina. Furthermore, we demonstrate that each of these variants is expressed at the protein level as assessed by Western blotting using splice-specific antibodies that we have generated. We conclude that EAAT5 exists in multiple spliced forms, and propose, based upon retention or absence of key structural features, that these variant forms may potentially exhibit distinct properties relative to the originally described form of EAAT5.

UR - http://www.scopus.com/inward/record.url?scp=84864548572&partnerID=8YFLogxK

U2 - 10.1016/j.gene.2012.07.010

DO - 10.1016/j.gene.2012.07.010

M3 - Article

VL - 506

SP - 283

EP - 288

JO - Gene

JF - Gene

SN - 0378-1119

IS - 2

ER -