Aflatoxin b1—its effects on an in vitro plant system

M. McLean; M. F. Dutton; M. P. Watt; P. Berjak

doi:10.1080/02652039509374327

Aflatoxin b₁—its effects on an in vitro plant system

M. McLean, M. F. Dutton, M. P. Watt, P. Berjak

Research output: Contribution to journal › Article › Research › peer-review

16 Citations (Scopus)

Abstract

The phytotoxic effects of aflatoxin B₁ (AFB₁) onin vitro cultures of differentiating calli and regenerating plantlets ofNicotiana tabacum were assessed. Callus appeared more sensitive to the effects of AFB₁, with fresh mass accumulation and callus chlorophyll levels affected at low (approximately 0.5 µg/ml) aflatoxin concentrations. Transmission electron microscopy revealed early deteriorative alterations in chloroplast morphology. Inhibitory effects of the toxin (up to and including 10 µg/ml) on callus fresh mass accumulation were reversed following a 3 week toxin-free recovery period. In tobacco plantlets, root and leaf development, and root and leaf mass were significantly inhibited in a dose-dependent fashion with increasing AFB₁ concentration above 0.5 µg/ml. Inhibitory effects on plantlet root development were more pronounced that on leaf development.

Original language	English
Pages (from-to)	435-443
Number of pages	9
Journal	Food Additives and Contaminants
Volume	12
Issue number	3
DOIs	https://doi.org/10.1080/02652039509374327
Publication status	Published - 1995
Externally published	Yes

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title = "Aflatoxin b1—its effects on an in vitro plant system",

abstract = "The phytotoxic effects of aflatoxin B1 (AFB1) onin vitro cultures of differentiating calli and regenerating plantlets ofNicotiana tabacum were assessed. Callus appeared more sensitive to the effects of AFB1, with fresh mass accumulation and callus chlorophyll levels affected at low (approximately 0.5 µg/ml) aflatoxin concentrations. Transmission electron microscopy revealed early deteriorative alterations in chloroplast morphology. Inhibitory effects of the toxin (up to and including 10 µg/ml) on callus fresh mass accumulation were reversed following a 3 week toxin-free recovery period. In tobacco plantlets, root and leaf development, and root and leaf mass were significantly inhibited in a dose-dependent fashion with increasing AFB1 concentration above 0.5 µg/ml. Inhibitory effects on plantlet root development were more pronounced that on leaf development.",

author = "M. McLean and Dutton, {M. F.} and Watt, {M. P.} and P. Berjak",

note = "Funding Information: The services ofthe Electron Microscopy Unit,Faculty ofMedicine, University of Natal, Durban, are duly acknowledged. Research wasmade possible through funding from the University of Natal.",

year = "1995",

doi = "10.1080/02652039509374327",

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pages = "435--443",

journal = "Food Additives and Contaminants",

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T1 - Aflatoxin b1—its effects on an in vitro plant system

AU - McLean, M.

AU - Dutton, M. F.

AU - Watt, M. P.

AU - Berjak, P.

N1 - Funding Information: The services ofthe Electron Microscopy Unit,Faculty ofMedicine, University of Natal, Durban, are duly acknowledged. Research wasmade possible through funding from the University of Natal.

PY - 1995

Y1 - 1995

N2 - The phytotoxic effects of aflatoxin B1 (AFB1) onin vitro cultures of differentiating calli and regenerating plantlets ofNicotiana tabacum were assessed. Callus appeared more sensitive to the effects of AFB1, with fresh mass accumulation and callus chlorophyll levels affected at low (approximately 0.5 µg/ml) aflatoxin concentrations. Transmission electron microscopy revealed early deteriorative alterations in chloroplast morphology. Inhibitory effects of the toxin (up to and including 10 µg/ml) on callus fresh mass accumulation were reversed following a 3 week toxin-free recovery period. In tobacco plantlets, root and leaf development, and root and leaf mass were significantly inhibited in a dose-dependent fashion with increasing AFB1 concentration above 0.5 µg/ml. Inhibitory effects on plantlet root development were more pronounced that on leaf development.

AB - The phytotoxic effects of aflatoxin B1 (AFB1) onin vitro cultures of differentiating calli and regenerating plantlets ofNicotiana tabacum were assessed. Callus appeared more sensitive to the effects of AFB1, with fresh mass accumulation and callus chlorophyll levels affected at low (approximately 0.5 µg/ml) aflatoxin concentrations. Transmission electron microscopy revealed early deteriorative alterations in chloroplast morphology. Inhibitory effects of the toxin (up to and including 10 µg/ml) on callus fresh mass accumulation were reversed following a 3 week toxin-free recovery period. In tobacco plantlets, root and leaf development, and root and leaf mass were significantly inhibited in a dose-dependent fashion with increasing AFB1 concentration above 0.5 µg/ml. Inhibitory effects on plantlet root development were more pronounced that on leaf development.

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EP - 443

JO - Food Additives and Contaminants

JF - Food Additives and Contaminants

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ER -

Aflatoxin b₁—its effects on an in vitro plant system

Abstract

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