A Stromal Organiser Cell that Directs Neonatal Spleen Regeneration

Jonathan Kah Huat Tan, Takeshi Watanabe

Research output: Contribution to conferencePresentationResearch

Abstract

Development of lymphoid tissue is determined by interactions
between stromal lymphoid tissue organiser (LTo) and hematopoietic lymphoid tissue inducer (LTi) cells. A failure for LTo to receive appropriate activating signals during embryogenesis through lymphotoxin engagement leads to a complete cessation of lymph node (LN) and Peyer’s patch development, identifying LTo as a key stromal population for lymphoid tissue organogenesis. However, little is known about the equivalent stromal cells that induce spleen development. Here, by dissociating neonatal murine spleen stromal tissue for re-aggregation and transplant into adult mouse recipients, we have identified a MAdCAM- 1+CD31+CD201+ spleen stromal organizer cell-type critical for new tissue formation. This finding provides an insight into the regulation of post-natal spleen tissue organogenesis, and could be exploited in the development of spleen regenerative therapies.
Original languageEnglish
Pages29
Number of pages1
Publication statusPublished - 13 Nov 2016
EventAustralian Society of Medical Research National Scientific Conference - Bond University, Gold Coast, Australia
Duration: 13 Nov 201615 Nov 2016
Conference number: 53rd

Conference

ConferenceAustralian Society of Medical Research National Scientific Conference
CountryAustralia
CityGold Coast
Period13/11/1615/11/16

Fingerprint

Lymphoid Tissue
Stromal Cells
Regeneration
Spleen
Organogenesis
Lymphotoxin-alpha
Peyer's Patches
Helper-Inducer T-Lymphocytes
Embryonic Development
Lymph Nodes
Transplants
Population

Cite this

Tan, J. K. H., & Watanabe, T. (2016). A Stromal Organiser Cell that Directs Neonatal Spleen Regeneration. 29. Australian Society of Medical Research National Scientific Conference, Gold Coast, Australia.
Tan, Jonathan Kah Huat ; Watanabe, Takeshi. / A Stromal Organiser Cell that Directs Neonatal Spleen Regeneration. Australian Society of Medical Research National Scientific Conference, Gold Coast, Australia.1 p.
@conference{b8f46c02a31e45b78f9454b55f5b3e9b,
title = "A Stromal Organiser Cell that Directs Neonatal Spleen Regeneration",
abstract = "Development of lymphoid tissue is determined by interactionsbetween stromal lymphoid tissue organiser (LTo) and hematopoietic lymphoid tissue inducer (LTi) cells. A failure for LTo to receive appropriate activating signals during embryogenesis through lymphotoxin engagement leads to a complete cessation of lymph node (LN) and Peyer’s patch development, identifying LTo as a key stromal population for lymphoid tissue organogenesis. However, little is known about the equivalent stromal cells that induce spleen development. Here, by dissociating neonatal murine spleen stromal tissue for re-aggregation and transplant into adult mouse recipients, we have identified a MAdCAM- 1+CD31+CD201+ spleen stromal organizer cell-type critical for new tissue formation. This finding provides an insight into the regulation of post-natal spleen tissue organogenesis, and could be exploited in the development of spleen regenerative therapies.",
author = "Tan, {Jonathan Kah Huat} and Takeshi Watanabe",
year = "2016",
month = "11",
day = "13",
language = "English",
pages = "29",
note = "Australian Society of Medical Research National Scientific Conference ; Conference date: 13-11-2016 Through 15-11-2016",

}

Tan, JKH & Watanabe, T 2016, 'A Stromal Organiser Cell that Directs Neonatal Spleen Regeneration' Australian Society of Medical Research National Scientific Conference, Gold Coast, Australia, 13/11/16 - 15/11/16, pp. 29.

A Stromal Organiser Cell that Directs Neonatal Spleen Regeneration. / Tan, Jonathan Kah Huat; Watanabe, Takeshi.

2016. 29 Australian Society of Medical Research National Scientific Conference, Gold Coast, Australia.

Research output: Contribution to conferencePresentationResearch

TY - CONF

T1 - A Stromal Organiser Cell that Directs Neonatal Spleen Regeneration

AU - Tan, Jonathan Kah Huat

AU - Watanabe, Takeshi

PY - 2016/11/13

Y1 - 2016/11/13

N2 - Development of lymphoid tissue is determined by interactionsbetween stromal lymphoid tissue organiser (LTo) and hematopoietic lymphoid tissue inducer (LTi) cells. A failure for LTo to receive appropriate activating signals during embryogenesis through lymphotoxin engagement leads to a complete cessation of lymph node (LN) and Peyer’s patch development, identifying LTo as a key stromal population for lymphoid tissue organogenesis. However, little is known about the equivalent stromal cells that induce spleen development. Here, by dissociating neonatal murine spleen stromal tissue for re-aggregation and transplant into adult mouse recipients, we have identified a MAdCAM- 1+CD31+CD201+ spleen stromal organizer cell-type critical for new tissue formation. This finding provides an insight into the regulation of post-natal spleen tissue organogenesis, and could be exploited in the development of spleen regenerative therapies.

AB - Development of lymphoid tissue is determined by interactionsbetween stromal lymphoid tissue organiser (LTo) and hematopoietic lymphoid tissue inducer (LTi) cells. A failure for LTo to receive appropriate activating signals during embryogenesis through lymphotoxin engagement leads to a complete cessation of lymph node (LN) and Peyer’s patch development, identifying LTo as a key stromal population for lymphoid tissue organogenesis. However, little is known about the equivalent stromal cells that induce spleen development. Here, by dissociating neonatal murine spleen stromal tissue for re-aggregation and transplant into adult mouse recipients, we have identified a MAdCAM- 1+CD31+CD201+ spleen stromal organizer cell-type critical for new tissue formation. This finding provides an insight into the regulation of post-natal spleen tissue organogenesis, and could be exploited in the development of spleen regenerative therapies.

M3 - Presentation

SP - 29

ER -

Tan JKH, Watanabe T. A Stromal Organiser Cell that Directs Neonatal Spleen Regeneration. 2016. Australian Society of Medical Research National Scientific Conference, Gold Coast, Australia.