A sensitive and quantitative fluorescence assay for cell surface antigens

Terry J Higgins; Helen C O'Neill; Christopher R Parish

doi:10.1016/0022-1759(81)90283-0

A sensitive and quantitative fluorescence assay for cell surface antigens

Terry J Higgins
, Helen C O'Neill
, Christopher R Parish

Research output: Contribution to journal › Article › Research › peer-review

10 Citations (Scopus)

Abstract

A procedure is described for the assay of cell surface antigens based on quantitative fluorometry. Fluorescent immunospheres are coupled with sheep anti-mouse immunoglobulins or Protein A and used to detect specific antibody bound to target cells. The fluorescent sphere assay described here offers 16--128-fold greater sensitivity than complement mediated lysis or Protein A radioimmune assays and comparable sensitivity to rosetting assays. In addition, the assay is simple to perform, uses commercially available reagents and is completely objective in that a common laboratory fluorometer is used to obtain fluorescence measurements.

Original language	English
Pages (from-to)	275-287
Number of pages	13
Journal	Journal of Immunological Methods
Volume	47
Issue number	3
DOIs	https://doi.org/10.1016/0022-1759(81)90283-0
Publication status	Published - 1981
Externally published	Yes

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title = "A sensitive and quantitative fluorescence assay for cell surface antigens",

abstract = "A procedure is described for the assay of cell surface antigens based on quantitative fluorometry. Fluorescent immunospheres are coupled with sheep anti-mouse immunoglobulins or Protein A and used to detect specific antibody bound to target cells. The fluorescent sphere assay described here offers 16--128-fold greater sensitivity than complement mediated lysis or Protein A radioimmune assays and comparable sensitivity to rosetting assays. In addition, the assay is simple to perform, uses commercially available reagents and is completely objective in that a common laboratory fluorometer is used to obtain fluorescence measurements.",

author = "Higgins, \{Terry J\} and O'Neill, \{Helen C\} and Parish, \{Christopher R\}",

year = "1981",

doi = "10.1016/0022-1759(81)90283-0",

language = "English",

volume = "47",

pages = "275--287",

journal = "Journal of Immunological Methods",

issn = "0022-1759",

publisher = "CA: Journal of Cultural Analytics",

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TY - JOUR

T1 - A sensitive and quantitative fluorescence assay for cell surface antigens

AU - Higgins, Terry J

AU - O'Neill, Helen C

AU - Parish, Christopher R

PY - 1981

Y1 - 1981

N2 - A procedure is described for the assay of cell surface antigens based on quantitative fluorometry. Fluorescent immunospheres are coupled with sheep anti-mouse immunoglobulins or Protein A and used to detect specific antibody bound to target cells. The fluorescent sphere assay described here offers 16--128-fold greater sensitivity than complement mediated lysis or Protein A radioimmune assays and comparable sensitivity to rosetting assays. In addition, the assay is simple to perform, uses commercially available reagents and is completely objective in that a common laboratory fluorometer is used to obtain fluorescence measurements.

AB - A procedure is described for the assay of cell surface antigens based on quantitative fluorometry. Fluorescent immunospheres are coupled with sheep anti-mouse immunoglobulins or Protein A and used to detect specific antibody bound to target cells. The fluorescent sphere assay described here offers 16--128-fold greater sensitivity than complement mediated lysis or Protein A radioimmune assays and comparable sensitivity to rosetting assays. In addition, the assay is simple to perform, uses commercially available reagents and is completely objective in that a common laboratory fluorometer is used to obtain fluorescence measurements.

U2 - 10.1016/0022-1759(81)90283-0

DO - 10.1016/0022-1759(81)90283-0

M3 - Article

C2 - 7037966

SN - 0022-1759

VL - 47

SP - 275

EP - 287

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

IS - 3

ER -

A sensitive and quantitative fluorescence assay for cell surface antigens

Abstract

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