Abstract
A procedure is described for the assay of cell surface antigens based on quantitative fluorometry. Fluorescent immunospheres are coupled with sheep anti-mouse immunoglobulins or Protein A and used to detect specific antibody bound to target cells. The fluorescent sphere assay described here offers 16--128-fold greater sensitivity than complement mediated lysis or Protein A radioimmune assays and comparable sensitivity to rosetting assays. In addition, the assay is simple to perform, uses commercially available reagents and is completely objective in that a common laboratory fluorometer is used to obtain fluorescence measurements.
| Original language | English |
|---|---|
| Pages (from-to) | 275-287 |
| Number of pages | 13 |
| Journal | Journal of Immunological Methods |
| Volume | 47 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 1981 |
| Externally published | Yes |