A sensitive and quantitative fluorescence assay for cell surface antigens

Terry J Higgins, Helen C O'Neill, Christopher R Parish

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Abstract

A procedure is described for the assay of cell surface antigens based on quantitative fluorometry. Fluorescent immunospheres are coupled with sheep anti-mouse immunoglobulins or Protein A and used to detect specific antibody bound to target cells. The fluorescent sphere assay described here offers 16--128-fold greater sensitivity than complement mediated lysis or Protein A radioimmune assays and comparable sensitivity to rosetting assays. In addition, the assay is simple to perform, uses commercially available reagents and is completely objective in that a common laboratory fluorometer is used to obtain fluorescence measurements.

Original languageEnglish
Pages (from-to)275-287
Number of pages13
JournalJournal of Immunological Methods
Volume47
Issue number3
DOIs
Publication statusPublished - 1981
Externally publishedYes

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Staphylococcal Protein A
Surface Antigens
Fluorescence
Fluorometry
Immunoglobulin A
Sheep
Antibodies

Cite this

Higgins, Terry J ; O'Neill, Helen C ; Parish, Christopher R. / A sensitive and quantitative fluorescence assay for cell surface antigens. In: Journal of Immunological Methods. 1981 ; Vol. 47, No. 3. pp. 275-287.
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A sensitive and quantitative fluorescence assay for cell surface antigens. / Higgins, Terry J; O'Neill, Helen C; Parish, Christopher R.

In: Journal of Immunological Methods, Vol. 47, No. 3, 1981, p. 275-287.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

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AU - Higgins, Terry J

AU - O'Neill, Helen C

AU - Parish, Christopher R

PY - 1981

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N2 - A procedure is described for the assay of cell surface antigens based on quantitative fluorometry. Fluorescent immunospheres are coupled with sheep anti-mouse immunoglobulins or Protein A and used to detect specific antibody bound to target cells. The fluorescent sphere assay described here offers 16--128-fold greater sensitivity than complement mediated lysis or Protein A radioimmune assays and comparable sensitivity to rosetting assays. In addition, the assay is simple to perform, uses commercially available reagents and is completely objective in that a common laboratory fluorometer is used to obtain fluorescence measurements.

AB - A procedure is described for the assay of cell surface antigens based on quantitative fluorometry. Fluorescent immunospheres are coupled with sheep anti-mouse immunoglobulins or Protein A and used to detect specific antibody bound to target cells. The fluorescent sphere assay described here offers 16--128-fold greater sensitivity than complement mediated lysis or Protein A radioimmune assays and comparable sensitivity to rosetting assays. In addition, the assay is simple to perform, uses commercially available reagents and is completely objective in that a common laboratory fluorometer is used to obtain fluorescence measurements.

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DO - 10.1016/0022-1759(81)90283-0

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SP - 275

EP - 287

JO - Immunotechnology

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