A sensitive and quantitative fluorescence assay for cell surface antigens

Terry J Higgins, Helen C O'Neill, Christopher R Parish

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10 Citations (Scopus)

Abstract

A procedure is described for the assay of cell surface antigens based on quantitative fluorometry. Fluorescent immunospheres are coupled with sheep anti-mouse immunoglobulins or Protein A and used to detect specific antibody bound to target cells. The fluorescent sphere assay described here offers 16--128-fold greater sensitivity than complement mediated lysis or Protein A radioimmune assays and comparable sensitivity to rosetting assays. In addition, the assay is simple to perform, uses commercially available reagents and is completely objective in that a common laboratory fluorometer is used to obtain fluorescence measurements.

Original languageEnglish
Pages (from-to)275-287
Number of pages13
JournalJournal of Immunological Methods
Volume47
Issue number3
DOIs
Publication statusPublished - 1981
Externally publishedYes

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