A role for early cytotoxic T cells in resistance to ectromelia virus infection in mice

H C O'Neill, Mary Brenan

Research output: Contribution to journalArticleResearchpeer-review

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Abstract

Ectromelia virus-specific cytotoxic T (Tc) cell precursors were present in the draining popliteal lymph node of all strains of mice tested at 2 to 3 days after footpad inoculation of a high dose (10(5) p.f.u.) of the virulent Moscow strain of ectromelia virus. To detect this response it was necessary to culture lymph node cells from infected mice in the presence of T cell growth factors and to use the more sensitive neutral red assay for measuring cytotoxicity. Cells with lytic activity were virus-specific, major histocompatibility complex-restricted TC cells. C57BL/6J resistant mice, which express a single dominant gene conferring innate resistance had virus-specific TC cell precursors 1 to 2 days sooner than did susceptible BALB/b mice. This TC cell-mediated immune response early after infection could account for the barrier to virus dissemination known to operate 1 to 2 days after infection to slow virus passage into the lymphoreticular system.

Original languageEnglish
Pages (from-to)2669-2673
Number of pages5
JournalJournal of General Virology
Volume68
Issue number10
DOIs
Publication statusPublished - Oct 1987
Externally publishedYes

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Ectromelia virus
Virus Diseases
T-Lymphocytes
Viruses
Lymph Nodes
T-Lymphoid Precursor Cells
Neutral Red
Dominant Genes
Moscow
Infection
Major Histocompatibility Complex
Interleukin-2

Cite this

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abstract = "Ectromelia virus-specific cytotoxic T (Tc) cell precursors were present in the draining popliteal lymph node of all strains of mice tested at 2 to 3 days after footpad inoculation of a high dose (10(5) p.f.u.) of the virulent Moscow strain of ectromelia virus. To detect this response it was necessary to culture lymph node cells from infected mice in the presence of T cell growth factors and to use the more sensitive neutral red assay for measuring cytotoxicity. Cells with lytic activity were virus-specific, major histocompatibility complex-restricted TC cells. C57BL/6J resistant mice, which express a single dominant gene conferring innate resistance had virus-specific TC cell precursors 1 to 2 days sooner than did susceptible BALB/b mice. This TC cell-mediated immune response early after infection could account for the barrier to virus dissemination known to operate 1 to 2 days after infection to slow virus passage into the lymphoreticular system.",
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A role for early cytotoxic T cells in resistance to ectromelia virus infection in mice. / O'Neill, H C; Brenan, Mary.

In: Journal of General Virology, Vol. 68 , No. 10, 10.1987, p. 2669-2673.

Research output: Contribution to journalArticleResearchpeer-review

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