Abstract
Introduction/Aim: Bladder dysfunction can lead to a range of debilitating conditions, and treatments are often associated with several side effects that substantially reduce quality of life. Research into alternative targets acting on bladder layers, including 5-hydroxytryptamine (5-HT, also termed serotonin) is extremely valuable. The aim of this study was to determine the effects of 5-HT on the contractile activity of the urothelium and lamina propria, and the likely mediating receptor.
Methods: Strips of porcine urothelium and lamina propria were suspended in 10mL organ baths filled with warm Krebs-bicarbonate solution. Cumulative dose-response curves using 5-Hydroxytryptamine were generated in the presence of the 5-HT antagonists, methiothepin, ketanserin, ondansetron and GR113808. Maximal tissue contractions were determined with carbachol doses. Data was analysed using a paired Student’s t-test.
Results: 5-HT application produced concentration-dependent contractions in the tissue. Maximal contractions to 5-HT (0.94±0.08g) were approximately 17% of carbachol-mediated contractions (n=37, 30µM). 5-HT stimulated contractions were unchanged in the presence of the nitric oxide synthesis inhibitor L-NNA (n=5, 100µM) or cyclooxygenase inhibitor indomethacin (n=4, 100µM). In the presence of the 5-HT1 antagonist responses were significantly (p?0.001) decreased (n=8, 10nM). Ketanserin (n=9, 30nM), ondansetron (n=13, 30nM) and GR113808 (n=8, 100nM) produced no significant changes in the 5-HT contractile response (all p=NSD).
Methods: Strips of porcine urothelium and lamina propria were suspended in 10mL organ baths filled with warm Krebs-bicarbonate solution. Cumulative dose-response curves using 5-Hydroxytryptamine were generated in the presence of the 5-HT antagonists, methiothepin, ketanserin, ondansetron and GR113808. Maximal tissue contractions were determined with carbachol doses. Data was analysed using a paired Student’s t-test.
Results: 5-HT application produced concentration-dependent contractions in the tissue. Maximal contractions to 5-HT (0.94±0.08g) were approximately 17% of carbachol-mediated contractions (n=37, 30µM). 5-HT stimulated contractions were unchanged in the presence of the nitric oxide synthesis inhibitor L-NNA (n=5, 100µM) or cyclooxygenase inhibitor indomethacin (n=4, 100µM). In the presence of the 5-HT1 antagonist responses were significantly (p?0.001) decreased (n=8, 10nM). Ketanserin (n=9, 30nM), ondansetron (n=13, 30nM) and GR113808 (n=8, 100nM) produced no significant changes in the 5-HT contractile response (all p=NSD).
| Original language | English |
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| Publication status | Published - 2015 |
| Event | The Gold Coast Health and Medical Research Conference The Gold Coast Health and Medical Research Conference The Gold Coast Health and Medical Research Conference - Gold Coast, Australia Duration: 3 Dec 2016 → 4 Dec 2016 |
Conference
| Conference | The Gold Coast Health and Medical Research Conference The Gold Coast Health and Medical Research Conference The Gold Coast Health and Medical Research Conference |
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| Country/Territory | Australia |
| City | Gold Coast |
| Period | 3/12/16 → 4/12/16 |