A new procedure for analysing the relationship between different cell surface antigens

Christopher R Parish; Helen C O'Neill; Ian F C McKenzie

doi:10.1016/0022-1759(80)90057-5

A new procedure for analysing the relationship between different cell surface antigens

Christopher R Parish, Helen C O'Neill, Ian F C McKenzie

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10 Citations (Scopus)

Abstract

An immunoprecipitation-inhibition procedure is assessing whether different antigenic determinants are carried on the same molecule or on different molecules on cells. The procedure entails (a) exposing NP-40 lysates of cells to antibody against one antigenic specificity; (b) removing free antibody and immune complexes by absorption with protein A-bearing S. aureus bacteria; (c) adsorption of the NP-40 with a detergent binding resin; and (d) measuring the inhibitory activity of the lysates for antibody against another specificity by a rosetting assay. This method has several advantages over the widely used sequential immunoprecipitation procedure.

Original language	English
Pages (from-to)	223-232
Number of pages	10
Journal	Journal of Immunological Methods
Volume	39
Issue number	3
DOIs	https://doi.org/10.1016/0022-1759(80)90057-5
Publication status	Published - 1980
Externally published	Yes

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title = "A new procedure for analysing the relationship between different cell surface antigens",

abstract = "An immunoprecipitation-inhibition procedure is assessing whether different antigenic determinants are carried on the same molecule or on different molecules on cells. The procedure entails (a) exposing NP-40 lysates of cells to antibody against one antigenic specificity; (b) removing free antibody and immune complexes by absorption with protein A-bearing S. aureus bacteria; (c) adsorption of the NP-40 with a detergent binding resin; and (d) measuring the inhibitory activity of the lysates for antibody against another specificity by a rosetting assay. This method has several advantages over the widely used sequential immunoprecipitation procedure.",

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AU - O'Neill, Helen C

AU - McKenzie, Ian F C

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AB - An immunoprecipitation-inhibition procedure is assessing whether different antigenic determinants are carried on the same molecule or on different molecules on cells. The procedure entails (a) exposing NP-40 lysates of cells to antibody against one antigenic specificity; (b) removing free antibody and immune complexes by absorption with protein A-bearing S. aureus bacteria; (c) adsorption of the NP-40 with a detergent binding resin; and (d) measuring the inhibitory activity of the lysates for antibody against another specificity by a rosetting assay. This method has several advantages over the widely used sequential immunoprecipitation procedure.

U2 - 10.1016/0022-1759(80)90057-5

DO - 10.1016/0022-1759(80)90057-5

M3 - Article

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VL - 39

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EP - 232

JO - Journal of Immunological Methods

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ER -

A new procedure for analysing the relationship between different cell surface antigens

Abstract

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