A new procedure for analysing the relationship between different cell surface antigens

Christopher R Parish, Helen C O'Neill, Ian F C McKenzie

Research output: Contribution to journalArticleResearchpeer-review

10 Citations (Scopus)

Abstract

An immunoprecipitation-inhibition procedure is assessing whether different antigenic determinants are carried on the same molecule or on different molecules on cells. The procedure entails (a) exposing NP-40 lysates of cells to antibody against one antigenic specificity; (b) removing free antibody and immune complexes by absorption with protein A-bearing S. aureus bacteria; (c) adsorption of the NP-40 with a detergent binding resin; and (d) measuring the inhibitory activity of the lysates for antibody against another specificity by a rosetting assay. This method has several advantages over the widely used sequential immunoprecipitation procedure.

Original languageEnglish
Pages (from-to)223-232
Number of pages10
JournalJournal of Immunological Methods
Volume39
Issue number3
DOIs
Publication statusPublished - 1980
Externally publishedYes

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Surface Antigens
Immunoprecipitation
Antibodies
Epitopes
Staphylococcal Protein A
Antigen-Antibody Complex
Detergents
Adsorption
Bacteria
Nonidet P-40

Cite this

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A new procedure for analysing the relationship between different cell surface antigens. / Parish, Christopher R; O'Neill, Helen C; McKenzie, Ian F C.

In: Journal of Immunological Methods, Vol. 39, No. 3, 1980, p. 223-232.

Research output: Contribution to journalArticleResearchpeer-review

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AU - McKenzie, Ian F C

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