The emotional and mental states of high-altitude construction workers (e.g., emotions and mental fatigue) are one of the critical factors affecting work performance (e.g., safety, health, construction quality, and productivity). To prevent undesired results from adverse emotional and mental states, active interventions for workers are important. Taking scaffolders on site as specific objects, a multicomponent (with two intervention sessions) and neurophysiological intervention during working intervals is proposed in this research. A sample of 10 participants is randomly assigned to either an intervention group or control group. Emotional and mental inducement is conducted in advance to simulate the states of scaffolders in normal working conditions. Then a simple, rapid, and active intervention, consisting of a progressive muscle relaxation session and a trigeminal nerve stimulation session, is applied to the experimental group in a lounge environment for 13 min. For the control group, a normal resting mode (i.e. sitting still) is adopted instead. During the experiment, a wearable electroencephalogram sensor is used to collect electrical signals from related brain regions of a subject. Based on corresponding indices, the emotional and mental states of subjects are indicated by electroencephalogram signals. The combined effects of the progressive muscle relaxation and trigeminal nerve stimulation sessions in adjusting adverse emotional and mental states of high-altitude construction workers are determined by statistical analysis.