The 2.4-km run and 20-m multistage fitness test (20MSFT) are common aerobic fitness tests in law enforcement. Previous research analyzed relationships between these tests in recruits, and indicated limited transferability due to disparate demands (direction changes and running intensity). However, the correlations between 2.4-km run and 20MSFT in recruits after academy training may be different,where recruits should be fitter and able to complete high-intensity running efforts. This study documented changes in and relationships between the 2.4-km run and 20MSFT in law enforcement recruits after academy training. Retrospective analysis on 6 academy classes (276 males and 50 females) from one agency was conducted. The 20 MSFT and 2.4-km run were completed before and after academy training. Estimated V̇O2max was derived from 2.4-km run time and the 20MSFT shuttle number.Paired-samples t-tests ascertained whether the 2.4-km run and 20MSFT improved after academy. Correlations and linear regression derived relationships between the 2.4-km run and 20MSFT. Recruits improved in the 2.4-km run and 20MSFT (p , 0.01). There were significant correlations between the 2.4-km run and 20MSFT for all recruits (r 520.49), males (r 520.48), and females (r 520.29). However, the regression equations for all recruits (r2 5 0.24), males (r2 5 0.23), and females (r2 5 0.08) were low. The results suggested that there were disparate aspects to the 2.4-km run and 20MSFT in law enforcement recruits after academy training. Although aerobic fitness improved, there are likely other aspects important to the 20MSFT not developed during academy (change-of-direction ability and high-intensity running). This could impact the between-test relationship.